Sequences from confirmation amplicon


Confirmation sequences for line 079B01 and hit At3g21190 are shown.
Line ID 079B01
Locus At3g21190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7433163
Primers used for PCR 08sg - 8474, show primer details
   
Sequence Name 30-K116218-0022-079-B01C-08sg
GenBank Accession LN499866 [GenBank]
Sequencing primer gene specific primer, 08sg: 5'-AACAACTCTAAAGTGGTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K116218-0022-079-B01C-08sg
CTCTCTCTTGTTTGTGTGGTTATTCTGCTAATCTTGGTTTAGATCACTGATGCTGTAATG
GTGGCTAAGCATCTTGGAGCAACTCTAGTACTTCCTGATATAAGAGGAAGCAAACCTGGT
GATGAAAGGTTAGGGTTGTTTTTTGTATGTTCATTTTTGGTTTACTAATTTGTTTCTTAG
ATTTCTAAACTAATTTTAACTGCTTTATCTAGGAATTTTGAAGATATCTATGATGCGGAT
AAGCTGATTAAAAGCTTGGAAAATGTCGTTAAAGTTGTGAAAAAATTGCCTGAAGAAGTT
TCTCTAAGGAACATGGCCATTGTGAAGGTCCCTACCAGAGTTACAGAAGATTACATTAAA
GAGCACATTGATCCCATCTTTAAATCTAAAGGAAACATTCGAGTTGCATCATACTTCCCT
TCTGTAAACTTGAGAAAATCCTCACAAGATGGCGAAACTGATCCTGTAGCCTGTTTAGCA
ATGTTTGGGTCTTTGGAGTTGCAACCTGAAGTGAATGCAGTGGCTGAATCAATGGTTGAG
CGGTTGAGGACTCATAGCCGTAAATCCGGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 06-K116217-0022-079-B01C-8409
GenBank Accession LN488964 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K116217-0022-079-B01C-8409
ATTTACCGGATTTACGGCTATGAGTCCTCAACCGCTCAACCATTGATTCAGCCACTGCAT
TCACTTCAGGTTGCAACTCCAAAGACCCAAACATTGCTAAACAGGCTACAGGATCAGTTT
CGCCATCTTGTGAGGATTTTCTCAAGTTTACAGAAGGGAAGTATGATGCAACTCGAATGT
TTCCTTTAGATTTAAAGATGGGATCAATGTGCTCTTTAATGTAATCTTCTGTAACTCTGG
TAGGGACCTTCACAATGGCCATGTTCCTTAGAGAAACTTCTTCAGGCAATTTTTTCACAA
CTTTAACGACATTTTCCAAGCTTTTAATCAGCTTATCCGCATCATAGATATCTTCAAAAT
TCCTAGATAAAGCAGTTAAAATTAGTTTAGAAATCTAAGAAACAAATTAGTAAACCAAAA
ATGAACATACAAAAAACAACCCTAACCTTTCATCACCAGGTTTGCTTCCTCTTATATCAG
GAAGTACTAGAGTTGCTCCAAGATGCTTAGCCACCATTACAGCATCAGTGATCTAAACCA
AGATTAGCAGAATTAACCACACAAACAAGAAGAAGAAGAAGATAACAACACATCTNAAGA
ACACCACTT

Last Updated on 06.12.2018 16:11