Sequences from confirmation amplicon


Confirmation sequences for line 079D12 and hit At5g46800 are shown.
Line ID 079D12
Locus At5g46800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18988957
Primers used for PCR IL16 - 8474, show primer details
   
Sequence Name 21-K032414-022-079-D12C-8409
GenBank Accession LN495557 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K032414-022-079-D12C-8409
TTACAATTGAATATTGGCGTCATGAAGAACACTCTTCACAACATCTGTCGGATATACTAT
TCCCCAAAAAGAAGCACCAGCGACTCCACCCGCCATGATCAATGACCCTTGTCCTAAGCT
TGAAGTGTCTGAACCACCGGCTAAAAACCGCTTGAAAGCTTCGTAGGCTGCAAACATTGT
TGCATTTCCCGGGACTTCACGGGCGAATGTAGGGAATAAACCTTTGAATAGACCTCGTGC
TCCTCCTTCTGATCGTAGGACATGACGGGCTACATCCATTGGTCCACCGTATTTCACGGC
TGCAACCACTGAGCTTGTAGTAGAGGCACCAGCTAATGCACCTTGTGCTTGCAACCTGAT
GGAGAAACAAGATTAAACACACACACAAAGAGACATGTTTTAGTTCTGTTTCAGTCTAGA
GCTTGAGTTAAAAGCACATGTATATATAGGCTCTGATTGGTAATACATGTAAAAAGAACC
TAA
   
Sequence Name 21-K032414-022-079-D12C-IL16
GenBank Accession LN495558 [GenBank]
Sequencing primer gene specific primer, IL16: 5'-TCAAGTGCAGGTTAGGTTCTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K032414-022-079-D12C-IL16
ATGTGCTTTAACTCAGCTCTAGACTGAAACAGAACTAAAACATGTCTCTTTGTGTGTGTG
TTTAATCTTGTTTCTCCATCAGGTTGCAAGCACAAGGTGCATTAGCTGGTGCCTCTACTA
CAAGCTCAGTGGTTGCAGCCGTGAAATACGGTGGACCAATGGATGTAGCCCGTCATGTCC
TACGATCAGAAGGAGGAGCACGAGGTCTATTCAAAGGTTTATTCCCTACATTCGCCCGTG
AAGTCCCGGGAAATGCAACAATGTTTGCAGCCTACGAAGCTTTCAAGCGGTTTTTAGCCG
GTGGTTCAGACACTTCAAGCTTAGGACAAGGGTCATTGATCATGGCGGGTGGAGTCGCTG
GTGCTTCTTTTTGGGGAATAGTATATCCGACAGATGTTGTGAAGAGTGTTCTTCATTGAC
GCCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACTC

Last Updated on 06.12.2018 16:11