Sequences from confirmation amplicon


Confirmation sequences for line 079G05 and hit At2g02560 are shown.
Line ID 079G05
Locus At2g02560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:689825
Primers used for PCR CK66 - 8474, show primer details
   
Sequence Name 13-K024722-022-079-G05C-8409
GenBank Accession LN491868 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K024722-022-079-G05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACTTTCCTCAGAGCTCTCTCCTCTCCGCAACTT
CCACCACCACCAAATCGGAACTCTCGGCGTTCTACTCCGTCTCCGGCGTTACCTCCGTCG
ATTTCATCTTCTCAGGTTACTTAGGGTTACCTCTTTTTTGCTTTTTGATCGATAAAGTGT
GATGATGTCTAGCGTTAACTCTTTTCGTCTTCGTAGTCGTAGTCTTAGGGTTTTGGTGGC
GACGATACTTTGATTTATCAAACCGAACTTCTTCTTTGTGATCTTCGATGAATCAAAAGT
TTTCTAAGGGATATTAATTAGGTTTTGTTACCGGCGATAATTAATCCATCTTCGATGCTT
CTTCTTTACACTTCTGACTTTGTTTCTGATGTTTTAACCTCTGTGGAGAGCTTGATAGAG
TG
   
Sequence Name 14-K024722-022-079-G05C-CK66
GenBank Accession LN492338 [GenBank]
Sequencing primer gene specific primer, CK66: 5'-CAGAGCAATGTCACTCTATCAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K024722-022-079-G05C-CK66
CACAGAGGTTAAAACATCAGAAACAAAGTCAGAAGTGTAAAGAAGAAGCATCGAAGATGG
ATTAATTATCGCCGGTAACAAAACCTAATTAATATCCCTTAGAAAACTTTTGATTCATCG
AAGATCACAAAGAAGAAGTTCGGTTTGATAAATCAAAGTATCGTCGCCACCAAAACCCTA
AGACTACGACTACGAAGACGAAAAGAGTTAACGCTAGACATCATCACACTTTATCGATCA
AAAAGCAAAAAAGAGGTAACCCTAAGTAACCTGAGAAGATGAAATCGACGGAGGTAACGC
CGGAGACGGAGTAGAACGCCGAGAGTTCCGATTTGGTGGTGGTGGAAGTTGCGGAGAGGA
GAGAGCTCTGAGGAAAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTT
ATTTCGACGTGTCTCATTCA

Last Updated on 06.12.2018 16:11