Sequences from confirmation amplicon


Confirmation sequences for line 079H08 and hit At1g05260 are shown.
Line ID 079H08
Locus At1g05260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1531245
Primers used for PCR 21by - 8474, show primer details
   
Sequence Name 72-K108065-0022-079-H08C-21by
GenBank Accession LN512737 [GenBank]
Sequencing primer gene specific primer, 21by: 5'-TTATGATGGTTTCACAATAACCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K108065-0022-079-H08C-21by
AGAAATTAGAAAGTACTGTTTTTATGATATAGAGAACATTTCAACCTAGAGTTTCAAGAT
ATATTTACAAGAAATATATGTGTTATAAACAGGGGCTCACACTATTGGTGTATCTCACTG
CTCGTCTTTCACAAACCGTCTCTACAATTTTACGGGTCGTGGAGGCCAAGATCCGGCTTT
GGACAGCGAGTACGCAGCCAATCTCAAGTCTAGAAAATGTCCTAGCCTCAACGATAACAA
GACCATCGTAGAGATGGATCCAGGGAGCCGCAAAACATTTGATCTAAGTTATTACCAGCT
CGTACTCAAGCGTAGAGGTCTGTTTCAATCAGACTCTGCTCTCACCACTAACCCCACAAC
ACTTTCAAACATAAACCGGATCTTGACGGGTTCGGTGGGGAGTTTCTTCTCTGAGTTTGC
CAAGTCAATGGAGAAAATGGGTCGGATCAATGTCAAGACTGGTTCAGCTGGAGTGGTTAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTA
   
Sequence Name 02-K108066-0022-079-H08C-8409
GenBank Accession LN487064 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K108066-0022-079-H08C-8409
GCCATTTACAATTGAATTATCCTAACCACTCCAGCTGAACCAGTCTTGACATTGATCCGA
CCCATTTTCTCCATTGACTTGGCAAACTCAGAGAAGAAACTCCCCACCGAACCCGTCAAG
ATCCGGTTTATGTTTGAAAGTGTTGTGGGGTTAGTGGTGAGAGCAGAGTCTGATTGAAAC
AGACCTCTACGCTTGAGTACGAGCTGGTAATAACTTAGATCAAATGTTTTGCGGCTCCCT
GGATCCATCTCTACGATGGTCTTGTTATCGTTGAGGCTAGGACATTTTCTAGACTTGAGA
TTGGCTGCGTACTCGCTGTCCAAAGCCGGATCTTGGCCTCCACGACCCGTAAAATTGTAG
AGACGGTTTGTGAAAGACGAGCAGTGAGATACACCAATAGTGTGAGCCCCTGTTTATAAC
ACATATATTTCTTGTAAATATATCTTGAAACTCTAGGTTGAAATGTTCTCTATATCATAA
AAACAGTACTTTCTAATTTTCTTTATTGAAATCATAATAAGTATTACTTTTCGGTTATTG
TGAAACCATCATA

Last Updated on 06.12.2018 16:11