Sequences from confirmation amplicon


Confirmation sequences for line 081C12 and hit At5g10450 are shown.
Line ID 081C12
Locus At5g10450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3284446
Primers used for PCR IF25 - 8474, show primer details
   
Sequence Name 12-K032413-022-081-C12C-8409
GenBank Accession LN491459 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K032413-022-081-C12C-8409
ATCTGCTCCTGTTCATAGAGTAGTAATAACTCAGCACACACGGTTTTGCGGTTATTTCAG
TTTTAACATGTTCTTTGATGTCTCTTATATGATGCATCTGATTGGTCTATGATGGAAACA
CTAATCACACTAAGATATGATCTTTATAGTTATTCGAATGGTGTAGAATATCGAGGTACG
GCATAAAGAGATGTGAATACCTGCATATCGGAGGTCCAAAGGGTTAAATTGTCCCTCAGC
AACTGCATTATGAGAGTGCTGTCTTTGTAGGATTCCTCTCCCAGAGTGTCAAGCTCAGCT
ATGGCCTCCTCAAAAGCCTACAATTAAAAATCCAGTATCAAAATTGAGAATGCCCCCACA
GTGAAAAATTTCTGCCGTTTTACTAAATGTCTTATACGTAAGTTCTAGTTGAAACAGAAT
GAGACATCAGTCCTAGTATTGGACAACATGGATCTTCATCATCATATTAGTCTGTGATTA
TTAAAAAAGAGGAATTTTGTTAAAAAAGCTGACTCAAAGTGATACTAGATGAA
   
Sequence Name 12-K032413-022-081-C12C-IF25
GenBank Accession LN491460 [GenBank]
Sequencing primer gene specific primer, IF25: 5'-TTGATTCATGGTGCTTCATCTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K032413-022-081-C12C-IF25
CAAATCCTCTTTTTAATAATCACAGACTAATATGATGATGAAGATCCATGTTGTCCAATA
CTAGGACTGATGTCTCATTCTGTTTCAACTAGAACTTACGTATAAGACATTTAGTAAAAC
GGCAGAAATTTTTCACTGTGGGGGCATTCTCAATTTTGATACTGGATTTTTAATTGTAGG
CTTTTGAGGAGGCCATAGCTGAGCTTGACACTCTGGGAGAGGAATCCTACAAAGACAGCA
CTCTCATAATGCAGTTGCTGAGGGACAATTTAACCCTTTGGACCTCCGATATGCAGGTAT
TCACATCTCTTTATGCCGTACCTCGATATTCTACACCATTCGAATAACTATAAAGATCAT
ATCTTAGTGTGATTAGTGTTTCCATCATAGACCAATCAGATGCATCATATAAGAGACATC
AAAGAACATGTTAAAACTGAAATAACCGCAAAACCGTGTGTGCTGAGTTATTACTACTCT
ATGTAACAGGAGCAGATGGACGAGGCCTGAGAAACCTACATGAATCAGCAATGAGTATGA
TGGATCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTAC
Primers used for PCR FP80 - 8474, show primer details
   
Sequence Name 47-K118546w-0022-081-C12C-FP80
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, FP80: 5'-AGCCTATGTTGCACAGATTTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118546w-0022-081-C12C-FP80
CGATTTTATTATTCCAGNTTTCCACCGACAAAAAATCCACGAATTTTGGGTTCCAACTTG
ATTTTGACGATACAGTAGAAGGATCATAATTTAACCAGAGGAGCCTTAAGGAGAAAGATG
TTTTTAAGGCTTGAGCAGAGTCGAGACAATAAATATTAAGAGCACAGAGGTTTAGTAGAG
TAGATAAATCATCAAGCTGTGCACAATCATAATCATCATAAACACTTCAATCTTTTTTCA
CAGTTTTTGAGCAAAGGTTATGGGGATTTTGAGATCGATTTGGTGGCAGAAACATCGCGT
AACAACCCTCCCCCCCTTCATCTACATGTAGATGGCAATTGAATATTTACAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTAT
T
   
Sequence Name 27-K118545w-0022-081-C12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118545w-0022-081-C12C-8409
CATTTACAATTGTAATATTCAATTGCCATCTACATGTAGATGAAGGGGGGGAGGGTTGTT
ACGCGATGTTTCTGCCACCAAATCGATCTCAAAATCCCCATAACCTTTGCTCAAAAACTG
TGAAAAAAGATTGAAGTGTTTATGATGATTATGATTGTGCACAGCTTGATGATTTATCTA
CTCTACTAAACCTCTGTGCTCTTAATATTTATTGTCTCGACTCTGCTCAAGCCTTAAAAA
CATCTTTCTCCTTAAGGCTCCTCTGGTTAAATTATGATCCTTCTACTGTATCGTCAAAAT
CAAGTTGGAACCCAAAATTCGTGGATTTTTTGTCGGTGTGAAACTGTGAATAATAAAAAT
CGTCAATGTTGTTACGTATATAAAATCCAGTCAAACCAAGAAAATCTGTGCAACATAGGC
T

Last Updated on 06.12.2018 16:11