Sequences from confirmation amplicon


Confirmation sequences for line 081F08 and hit At1g04780 are shown.
Line ID 081F08
Locus At1g04780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1341886
Primers used for PCR KM51 - 8474, show primer details
   
Sequence Name 15-K032921-022-081-F08C-8409
GenBank Accession LN492866 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K032921-022-081-F08C-8409
GATGCTACTCCGCTGCCTCTTCTCGTCTTCCGTCAGATATCTCCAAGATCCTCGCTTTCT
GTGTCGTTTTCTTGATTAGTGTTTGAGAAAAGCTCCTCATCAGTCATTGCACCCGGTACT
CTTCTTGATTTTATGCTAACAACTACATTGTGCATATCATACACTTTTGCTTTCCATTGC
CCAACCATTTCTGTCTTCTCTTGACGCCTCCATGTTAATTGCGGGAAGAGAACAGCCTGA
GTAACATCGATCCCGGGTCTGAAAATGCTTGTTTTGGACATTGCAGCCACTTCTTGACGA
ACTTCTTCTTCTGATGCGGCAGCTCCAGCACCGTCTAGAGCATTCATGATCTCCTTATCC
TTATGTGATATCATAAGAAGAGAACCGGAAGGAACTTTACCATCCTCTGACCCATCTCCA
AGAAAAAGTATAGTCTGATCCGATCTTTGAATCCTGAAACCATCAAACCCAGCCAGTGTC
ATATCCGCTCTAAGATTCGCACCCCTCTTCCAAATCTTATAAGTATCCGAAGGCGCCACT
CTAGAAATGAAAGGTATAACAGAACTCTCG
   
Sequence Name 15-K032921-022-081-F08C-KM51
GenBank Accession LN492867 [GenBank]
Sequencing primer gene specific primer, KM51: 5'-GATCACTTTCCATTTCGAGAGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K032921-022-081-F08C-KM51
ATACTTATAGATTTGGAAGAGGGGTGCGAATCTTAGAGCGGATATGACACTGGCTGGGTT
TGATGGTTTCAGGATTCAAAGATCGGATCAGACTATACTTTTTCTTGGAGATGGGTCAGA
GGATGGTAAAGTTCCTTCCGGTTCTCTTCTTATGATATCACATAAGGATAAGGAGATCAT
GAATGCTCTAGACGGTGCTGGAGCTGCCGCATCAGAAGAAGAAGTTCGTCAAGAAGTGGC
TGCAATGTCCAAAACAAGCATTTTCAGACCCGGGATCGATGTTACTCAGGCTGTTCTCTT
CCCGCAATTAACATGGAGGCGTCAAGAGAAGACAGAAATGGTTGGGCAATGGAAAGCAAA
AGTGTATGATATGCACAATGTAGTTGTTAGCATAAAATCAAGAAGAGTACCGGGTGCAAT
GACTGATGAGGAGCTTTTCTCAAACACTAATCAAGAAAACGACACAGAAAGCGAGGATCT
TGGAGATATCTTGACGGAAGACGAGAAGAGGCAGCTGGAGTTAGCACTCAAGTTAAAAGA
ATATGATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
Primers used for PCR 20wp - 8474, show primer details
   
Sequence Name 48-K118893-0022-081-F08C-20wp
GenBank Accession LN506653 [GenBank]
Sequencing primer gene specific primer, 20wp: 5'-TTCACCGAAGAAGTTGGACTAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K118893-0022-081-F08C-20wp
CTCTTCAACTTTGTAATGTGACGAGTACTCTAATTGTAGGAATTACGGGAATAGCAACCT
GCAATCACAAATCCAAATGAAATGAGCAAAAAGCTGGACAATTATGATGATTCTGATCAT
AGAAGGTTATAAACAGACCTTGACTGGGAATGTTCCCGAAGGTAGTTTCGTGGTCATAAG
CTCTCTCAAGCGTCGGATTGCTTTGACTTTGTTTGCAAGGATATCGAGCAATGGAAGAAG
TTCTTTTGTCTGTAATGGGAACCTCTCAGAAAGCCAAAGAACTGGTCTAAGCCCTTTCTT
GTACTCATTCTCTTTGTTTCCTTCTTTTCGTTTGCTGCTTCCGCTGCCTTCTGATGTTGA
AGCTTTTGATGAATCTCTTAGCCCCCTGTTTTCGTTCCTCACAACTGTCTCAACCGTTGA
GTGTCTTCCCGGTTTTATCTGTCTTCCTCCACGAGATGGAGAATCATCGTCCCCAAGAAG
ATCACTTACTTTCTCATCAACACAAAGCGAGTTTCTTGGAGGAACACTACTTCGTCTGTG
CCCTTCCTCTCGTTTCCTCCAACCACTGAACCATCCTTTCTTCTCCTGTTTGCAATACCC
GTTCCCATCCGTTACTGGAATTTCTCGATCCTCGAAAGAACAACTATTCTGCTTTTGAGA
AATCCTAGAGCTCTCGCCGTTAGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 08-K118892-0022-081-F08C-oriL
GenBank Accession LN489949 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118892-0022-081-F08C-oriL
CATTTCATTGAATTATTCTAACGGCGAGAGCTCTAGGATTTCTCAAAAGCAGAATAGTTG
TTCTTTCGAGGATCGAGAAATTCCAGTAACGGATGGGAACGGGTATTGCAAACAGGAGAA
GAAAGGATGGTTCAGTGGTTGGAGGAAACGAGAGGAAGGGCACAGACGAAGTAGTGTTCC
TCCAAGAAACTCGCTTTGTGTTGATGAGAAAGTAAGTGATCTTCTTGGGGACGATGATTC
TCCATCTCGTGGAGGAAGACAGATAAAACCGGGAAGACACTCAACGGTTGAGACAGTTGT
GAGGAACGAAAACAGGGGGCTAAGAGATTCATCAAAAGCTTCAACATCAGAAGGCAGCGG
AAGCAGCAAACGAAAAGAAGGAAACAAAGAGAATGAGTACAAGAAAGGGCTTAGACCAGT
TCTTTGGCTTTCTGAGAGGTTCCCATTACAGACAAAAGAACTTCTTCCATTGCTCGATAT
CCTTGCAAACAAAGTCAAAGCAATCCGACGCTTGAGAGAGCTTATGACCACGAAACTACC
TTCGGGAACATTCCCAGTCAAGGTCTGTTTATAACCTTCTATGATCAGAATCATCATAAT
TGTCCAGCTTTTTGCTCATTTCATTTGGATTTGTGATTGCAGGTTGCTATTCCCGTAATT
CCTACAATTAGAGTACTCGTCACATTTACAAAGTTTGAAGAGCTAGAAGCTATCGAAGAT
GAGTTTGTTACACCGCCATCTAGTCCAACTTCTTTCGGTG
Primers used for PCR 10bg - 8474, show primer details
   
Sequence Name 47-K118893-0022-081-F08C-10bg
GenBank Accession LN506349 [GenBank]
Sequencing primer gene specific primer, 10bg: 5'-GTTTTATCTGTCTTCCTCCACGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118893-0022-081-F08C-10bg
CTTACTTTCTCATCACACAAAGCGAGTTTCTTGGAGGACACTACTTCGTCTGTGCCCTTC
CTCTCGTTTCCTCCAACCACTGAACCATCCTTTCTTCTCCTGTTTGCAATACCCGTTCCC
ATCCGTTACTGGAATTTCTCGATCCTCGAAAGAACAACTATTCTGCTTTTGAGAAATCCT
AGAGCTCTCGCCGTTAGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCATTATT
   
Sequence Name 07-K118892-0022-081-F08C-8409
GenBank Accession LN489492 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118892-0022-081-F08C-8409
TTTCATTGAATATATTCTAACGGCGAGAGCTCTAGGATTTCTCAAAAGCAGAATAGTTGT
TCTTTCGAGGATCGAGAAATTCCAGTAACGGATGGGAACGGGTATTGCAAACAGGAGAAG
AAAGGATGGTTCAGTGGTTGGAGGAAACGAGAGGAAGGGCACAGACGAAGTAGTGTTCCT
CCAAGAAACTCGCTTTGTGTTGATGAGAAAGTAAGTGATCTTCTTGGGGACGATGATTCT
CCATCTCGTGGAGGAAGACAGATAAAA

Last Updated on 06.12.2018 16:11