Sequences from confirmation amplicon


Confirmation sequences for line 081G07 and hit At1g70280 are shown.
Line ID 081G07
Locus At1g70280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26466349
Primers used for PCR 08cp - 8474, show primer details
   
Sequence Name 61-K108745-0022-081-G07C-8409
GenBank Accession LN510541 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K108745-0022-081-G07C-8409
ATTTACATTGAATATATCCACAGAAGCATCAGCATCAGCAACAACAGCCGCATAAGGATC
GAAGGCATTACTCATCGATCCCGCATACTTATTATGAGCAGGACAGTGAAAAGTCTAATG
AGATAGTCTTTGGAGCGGTCCAAGAACAGAGCAGCAAGCGTGTGGCTAAGCCTAAGCCGA
AGCCTATAGAGTCTGGAGATCAAATGAATAATAATACTCAACAGAATCTTCACTACAGAT
CTCATTCTGTTAGTTACCCATATGGATACTATCCTAATACATGAAAAGTCTCCGGTTTTG
TGCGTTTCTGAGATTATCAGTCTTCCTTAATAAGATTATGCAGGGAATTAAATTTTTTGG
GTATAGGTGTTTGTATTGGGGAATTTTAAGAGTATGCTCTCGTCTTATGAGAAATGTCTC
ATACTTTGATTGTATTGGGTAATATATATATGAAAAAATGATATGATTATCTTTGTGAAA
AAAGTGATTATGTACATTCCCTTGAGCTTGTTACAGCATTGTTAAATAAGTGTCCAACAT
AAGGTTTCTCATTTACTAGATTCATACTTACAACATCTTCGAAGTTTATAAAGTTCATTA
GATTTATCTTGAGAGTTTCATCGAAGGCTTTGTGTCGGTGGAACAAGGCGCAAAGAACGA
TAAGATGAATACAACCATGGCTG
   
Sequence Name 77-K108746-0022-081-G07C-08cp
GenBank Accession LN513563 [GenBank]
Sequencing primer gene specific primer, 08cp: 5'-TCAGCCATGGTTGTATTCATCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K108746-0022-081-G07C-08cp
GCCTTCGATGAACTCTCAGATAAATCTAATGAACTTTATAAACTTCCAAGAAGTGGTAAG
TATGAATCTAGTAAATGAGAAACCTTATGTTGGACACTTATTTAACAATGCTGTAACAAG
CTCAAGGGAATGTACATAATCACTTTTTTCACAAAGATAATCATATCATTTTTTCATATA
TATATTACCCAATACAATCAAAGTATGAGACATTTCTCATAAGACGAGAGCATACTCTTA
AAATTCCCCAATACAAACACCTATACCCAAAAAATTTAATTCCCTGCATAATCTTATTAA
GGAAGACTGATAATCTCAGAAACGCACAAAACCGGAGACTTTTCATGTATTAGGATAGTA
TCCATATGGGTAACTAACAGAATGAGATCTGTAGTGAAGATTCTGTTGAGTATTATTATT
CATTTGATCTCCAGACTCTATAGGCTTCGGCTTAGGCTTAGCCACACGCTTGCTGCTCTG
TTCTTGGACCGCTCCAAAGACTATCTCATTAGACTTTTCACTGTCCTGCTCATAATAAGT
ATGCGGGATCGATGAGTAATGCCTTCGATGCTGCTGCTGCTGTTGTTGCTGATGCTGATG
CTTCTGTTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGGTTATT

Last Updated on 06.12.2018 16:11