Sequences from confirmation amplicon


Confirmation sequences for line 081H01 and hit At1g60750 are shown.
Line ID 081H01
Locus At1g60750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22362527
Primers used for PCR 01ui - 8474, show primer details
   
Sequence Name 40-K117118-0022-081-H01C-01ui
GenBank Accession LN503846 [GenBank]
Sequencing primer gene specific primer, 01ui: 5'-TGCAAACTCGCACACCTATATACT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117118-0022-081-H01C-01ui
CTTCGCAAATTTTACGCGATTTGGCGAAGCGGTATACCCTCAAAACAGTTTCCAAACATG
TATTTTATCTTGCTTTCCAAGAAGACAATGGTGGAGTCTCTGAGTCTTTGTAAGTAACCA
TGTTGTTGTCATACCGTTCACCTTTCACGAAATCCGGTTGAGCAATGGCTTCAAGCTCAA
CCATCTCTTCAGGAGTGAGCTTCACGGATAAAGCTCCAATGTTTTGGTTGAGGTTTTGGA
TCTTGGAGGTTCCTGGAATTGGACATACATCATCTCCTTGATGGTGAACCCACGCTAGAT
TTTGAGTCCTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 18-K117117-0022-081-H01C-8409
GenBank Accession LN494479 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117117-0022-081-H01C-8409
GCATTTCATTGAAAAGGACTCAAAATCTAGCGTGGGGTTCACCATCAAGGAGATGATGTA
TGTCCAATTCCAGGAACCTCCAAGATCCAAAACCTCAACCAAAACATTGGAGCTTTATCC
GTGAAGCTCACTCCTGAAGAGATGGTTGAGCTTGAAGCCATTGCTCAACCGGATTTCGTG
AAAGGTGAACGGTATGACAACAACATGGTTACTTACAAAGACTCAGAGACTCCACCATTG
TCTTCTTGGAAAGCAAGATAAAATACATGTTTGGAAACTGTTTTGAGGGTATACCGCATT
CGCCAAATCGCGGTAAAATTGTGCTGAAGATGTATCTCTGTGTGTGTGAAATAAGTTAAG
TATATAGGTGTGGGAGTTTGCA

Last Updated on 06.12.2018 16:11