Sequences from confirmation amplicon


Confirmation sequences for line 081H10 and hit At4g35600 are shown.
Line ID 081H10
Locus At4g35600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16896322
Primers used for PCR DL44 - 8474, show primer details
   
Sequence Name 01-K018490-022-081-H10C-DL44
GenBank Accession LN486379 [GenBank]
Sequencing primer gene specific primer, DL44: 5'-ATTTCCATAAAGACACAAACGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K018490-022-081-H10C-DL44
GATGACTTTTAAGCATTTTGCTGGCACAAGCAAAAGTCTTTTTACTTCCTAACGGTTTCA
CATCTCTACTATTTTTTGAATGATGTGGACAGTGATTAAAAAAAAACATGTGAAATAAAA
TTACAAATAATTCAAAGATTTCCAAAGTAAAAACAAAATTCTCAATTATTTTTTTCAGAT
CACACGATCATAAATAGATAGAAAAGGCTCTTAGGCTATGATTCTTCTTTTTGGTCCCAT
CTTCTTGGCATTTTCGTTTGTTTTTTTTTAATTTCTTTTAAATGAGAAAGAAATGCATAA
AAAATGAAAAGGTAGACCAAAACCCTTCTTCGGTTCACGCTAGCAACCCCCAATTTTCCT
CCCTTTATTTTATTTTGTTATCTAAATTAAGTCAAAATTATTTTGCAACCCATCGTTGCC
TCTTTCAATAGTTCTAAACTATATATTCAATAGTCAATTTTCCTCCCTTTATTTTATTTT
GTTATCTAAATTAAGTCAAAATTATTTTGCAACCCATCGTTGCCTCTTTCAATAGTTCTA
AACTATATATTCAGTTGGTTCTCTTTCAATAGTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
   
Sequence Name 02-K018490-022-081-H10C-8409
GenBank Accession LN486832 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K018490-022-081-H10C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACTATTGAAAGAGAACC
AACTGAATATATAGTTTAGAACTATTGAAAGAGGCAACGATGGGTTGCAAAATAATTTTG
ACTTAATTTAGATAACAAAATAAAATAAAGGGAGGAAAATTGACTATTGAATATATAGTT
TAGAACTATTGAAAGAGGCAACGATGGGTTGCAAAATAATTTTGACTTAATTTAGATAAC
AAAATAAAATAAAGGGAGGAAAATTGGGGGTTGCTAGCGTGAACCGAAGAAGGGTTTTGG
TCTACCTTTTCATTTTTTATGCATTTCTTTCTCATTTAAAAGAAATTAAAAAAAAACAAA
CGAAAATGCCAAGAAGATGGGACCAAAAAGAAGAATCATAGCCTAAGAGCCTTTTCTATC
TNTTTATGATCGTGTGATCTGAAAAAAATAATTGAGAATTTTGTTTTTACTTTGGAAATC
TTTGAATTATTTGTAATTTTATTTCACATGTTTTTTTTTAATCACTGTCCACATCATTCA
AAAAATAGTAAAGATGTGAAACCGTTNGGAAGTAAAAAGACTTTTGCTTGTGCCAGCAAA
ATGCTTAAAAGTCATCCTCTCANTCACAGTGCTTGGACGTTTGTGT

Last Updated on 06.12.2018 16:11