Sequences from confirmation amplicon


Confirmation sequences for line 082B07 and hit At2g19730 are shown.
Line ID 082B07
Locus At2g19730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8512896
Primers used for PCR 04dr - 8474, show primer details
   
Sequence Name 24-K110307-0022-082-B07C-04dr
GenBank Accession LN497037 [GenBank]
Sequencing primer gene specific primer, 04dr: 5'-AACAAGCCTAAGCTCTCTGTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K110307-0022-082-B07C-04dr
CCAGGATGTCAAGGCTGTTGCTACCAGGTATTACTCAATTTTTATTACTTTAGTGTTTTT
TATATGTCTATGGTGTGCTTATAGGTCTCTATATCTAGTTTTGTGTGTTATTTTCACGGC
TATGATCTACAATTGGTTTTTATGTTCTTCCCTCTACCTGTGAGAGGAGAGTTAATATGT
TATGGTAAAACGATATGATGCATAGCTGTGCAAAGATTAAAATTTAACTTCATATAAGGC
TCCTTTTTGTGGCATATTGATGAAATATTAATTTTACACTTGGCTATTTTCATTGATCTG
CTTAATGTTGCTGTTTATTGGTTTCATACGTGAATACAATCTAAACCAAATGATTGTGTA
AATGTTTAGGTGGTGGACAACTACTACAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCAGCAGCGTTA
   
Sequence Name 08-K110306-0022-082-B07C-8409
GenBank Accession LN489779 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K110306-0022-082-B07C-8409
GCCATTTACAATTGAATATATCCTGTAGTAGTTGTCCACCACCTAAACATTTACACAATC
ATTTGGTTTAGATTGTATTCACGTATGAAACCAATAAACAGCAACATTAAGCAGATCAAT
GAAAATAGCCAAGTGTAAAATTAATATTTCATCAATATGCCACAAAAAGGAGCCTTATAT
GAAGTTAAATTTTAATCTTTGCACAGCTATGCATCATATCGTTTTACCATAACATATTAA
CTCTCCTCTCACAGGTAGAGGGAAGAACATAAAAACCAATTGTAGATCATAGCCGTGAAA
ATAACACACAAAACTAGATATAGAGACCTATAAGCACACCATAGACATATAAAAAACACT
AAAGTAATAAAAATTGAGTAATACCTGGTTAGCAACAGCCTTTGACATCCTGGGGAATTC
CTTCTTGAGGATAGACTTGTTGACAGAGAGCTTAGGCTTGT

Last Updated on 06.12.2018 16:11