Sequences from confirmation amplicon


Confirmation sequences for line 082E05 and hit At2g28680 are shown.
Line ID 082E05
Locus At2g28680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12303401
Primers used for PCR 22by - 3144, show primer details
   
Sequence Name 73-K108065-0022-082-E05C-22by
GenBank Accession LN512914 [GenBank]
Sequencing primer gene specific primer, 22by: 5'-AATCTTCACCGGTTTCTCTACTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K108065-0022-082-E05C-22by
CTTGTCGGATCTCAGACAGGTAACGGTATCGCGAAGGTTGATGCGAGCTTGAAGATGCCT
GAACCAAAGAAAGGTGACCGAAAAGGGTTTGTTTTGAACTGTTTGGAGGCTCCTCTTGAT
GTTGACATTAAGGATGGAGGAAGAGTTGTTGTGTTGAACACAAAGAATCTTCCTTTGGTT
GGTGAAGTTGGATTTGGAGCTGATCTTGTGAGAATCGATGGACACTCTATGTGTTCTCCT
GGATTCTCTTGTGACTCGGCTCTTCAAGTTACTTACATTGTTGGTGGAAGTGGTCGTGTT
CAGATTGTTGGTGCTGATGGGAAGAGAGTTCTTGAGACTCATGTGAAAGCTGGTGTTTTG
TTCATTGTTCCTAGGTTCTTTGTTGTCTCCAAGATTAATTATCAAACACTGATAGTTTAA
ACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGA
ACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTAC
GTCAGTGGAGATATCACACAAATCCA
   
Sequence Name 03-K108066-0022-082-E05C-3144
GenBank Accession LN487517 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K108066-0022-082-E05C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAT
AATTAATCTTGGAGACAACAAAGAACCTAGGAACAATGAACAAAACACCAGCTTTCACAT
GAGTCTCAAGAACTCTCTTCCCATCAGCACCAACAATCTGAACACGACCACTTCCACCAA
CAATGTAAGTAACTTGAAGAGCCGAGTCACAAGAGAATCCAGGAGAACACATAGAGTGTC
CATCGATTCTCACAAGATCAGCTCCAAATCCAACTTCACCAACCAAAGGAAGATTCTTTG
TGTTCAACACAACAACTCTTCCTCCATCCTTAATGTCAACATCAAGAGGAGCCTCCAAAC
AGTTCAAAACAAACCCTTTTCGGTCACCTTTCTTTGGTTCAGGCATCTTCAAGCTCGCAT
CAACCTTCACGATACCGTTACCTGTCTGAGATCCGACAAGCTTCTTCACGGTGGTCTCAT
CGAGATCCCAAGCTCTGCCAACAAACTCAGTAGAGAAA

Last Updated on 06.12.2018 16:11