Sequences from confirmation amplicon


Confirmation sequences for line 082G07 and hit At5g21170 are shown.
Line ID 082G07
Locus At5g21170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7205601
Primers used for PCR FQ89 - 8474, show primer details
   
Sequence Name 19-K116083-0022-082-G07C-FQ89
GenBank Accession LN494908 [GenBank]
Sequencing primer gene specific primer, FQ89: 5'-CAATATACAATGCGAGAAAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116083-0022-082-G07C-FQ89
GTGCATGAAATCACTCATGGCTTAGTGAATAGTGACCATCGCCTTTTACAAGTTTACATT
GGGCTTTGAAACCCTCCAAACTTTTCGAAACAATCTTTCGACCAATAACTAGTTTACGGG
ACTAACCAATACAAATCTTGGAAAATGATGATTCTTTGACCATCTCTATTCTAACCCCAC
CTATTGGAGTTTAAGGCCTTTTCATCGTCACGATACGCATACTATGATCACTGGCTCTAG
CAAGATGTTAAGGCACACAAATCGGTGGCAAGGAGGTGACTGGCCCTTGTGTCATTATCC
TTCTCAAAGGCATTGAACCAACTTTTTATTCACATTTGTTTATTATATACTAAAAGAAAT
AAACAAAAAAAAAGTGGATAATTATAAACTTTTGGACGCCAAGTCACTCGTTTATCCATT
TGGTACGGATTTTAATGATATCTCTCTCTCAAGAGCCCCTTTTATGCAAAATCTCTTTTT
CGCTACCAATGGCATTATTCGTAATTAACTTCTAACTTGAGGGGCGTTTCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CG
   
Sequence Name 03-K116082-0022-082-G07C-8409
GenBank Accession LN487631 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K116082-0022-082-G07C-8409
TTGAATATATCCTGAAACGCCCCTCAAGTTAGAAGTTAATTACGAATAATGCCATTGGTA
GCGAAAAAGAGATTTTGCATAAAAGGGGCTCTTGAGAGAGAGATATCATTAAAATCCGTA
CCAAATGGATAAACGAGTGACTTGGCGTCCAAAAGTTTATAATTATCCACTTTTTTTTTG
TTTATTTCTTTTAGTATATAATAAACAAATGTGAATAAAAAGTTGGTTCAATGCCTTTGA
GAAGGATAATGACACAAGGGCCAGTCACCTCCTTGCCACCGATTTGTGTGCCTTAACATC
TTGCTAGAGCCAGTGATCATAGTATGCGTATCGTGACGATAAAAAGGCCTTAAACTCCAA
TAGGTGGGGTTAGAATAGAGATGGTCAAAGAATCATCATTTTCCAAGATTTGTATTGGTT
AGTCCCGTAAACTAGTTATTGGTCGAAAGATTGTTTCGAAAAGTTTGGAGGGTTTCAAAG
CCAATGTAAACTTGTAAAAGGCGATGGTCACTATTCACTAAGCCATGAGTGATTGTCATG
CACTTTCCTTATAGGTTGAGTCATTGTTTTTTCTTTTGCTTTCTCGCTTGGTATAT

Last Updated on 06.12.2018 16:11