Sequences from confirmation amplicon


Confirmation sequences for line 083A06 and hit At4g26580 are shown.
Line ID 083A06
Locus At4g26580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13412522
Primers used for PCR KC39 - 8474, show primer details
   
Sequence Name 64-K035534-022-083-A06C-KC39
GenBank Accession LN511140 [GenBank]
Sequencing primer gene specific primer, KC39: 5'-CGAGGAAGCTACTAACGAGAGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K035534-022-083-A06C-KC39
TGGGAAGGAGTAACAGAGAGCGTTCCAAGCGAGTAGGGAGATGCAGAGGACATGAAGGAT
TGGAGCATAGTGGAAAGAACCAAACCGAGAATCAAAAACCCATACATTACCTATCACAAA
CCATATCGCAAAGAATAGCTCCCGCGATGTCCGGCATTTGTTCATCAAATGCGAGCACCT
AAAACACACACAAAAATAAGTCATTTTCCTAGAAATCTTTGCATTTTATGATCAAACTTG
AAAAAACTAGATTTTTGTTAGTAACCTGGTGGTCTCTTCCCTGCTTCTTTGTTGCTGCTC
AATATCGCCGAAGCTAAATGCATTTTCATGGCTAGTATCTTGCTGACGATACCGACCGTA
TAGTAACATAAGATTGAGCAGACATCCTACGTTGTAGCCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACTAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
   
Sequence Name 16-K035534-022-083-A06C-8409
GenBank Accession LN493347 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K035534-022-083-A06C-8409
GAGCCATTTAAATTGAATATATCCTGGCTACAACGTTGGATGTCTGCTCAATCTTATGTT
ACTATACGGTCGGTATCGTCAGCAAGATACTAGCCATGAAAATGCATTTAGCTTCGGCGA
TATTGAGCAGCAACAAAGAAGCAGGGAAGAGACCACCAGGTTACTAACAAAAATCTAGTT
TTTTCAAGTTTGATCATAAAATGCAAAGATTTCTAGGAAAATGACTTATTTTTGTGTGTG
TTTTAGGTGCTCGCATTTGATGAACAAATGCCGGACATCGCTGGAGCTATTCTTTGCGAT
ATGGTTTGTGATAGGTAATGTATGGGTTTTTGATTCTCGGTTTGGTTCTTTCCACTATGC
TCCAATCCTTCATGTCCTCTGCATCTCCCTACTCGCTTGGAACGCTCTCTGTTACTCCTT
CCCATTTCTTCTCTTCCTCCTGCTCTGTTGTGTTGTACCTCTCGTTAGTAGCTTCC

Last Updated on 06.12.2018 16:11