Sequences from confirmation amplicon


Confirmation sequences for line 084E02 and hit At1g49450 are shown.
Line ID 084E02
Locus At1g49450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18306885
Primers used for PCR BH86 - 8474, show primer details
   
Sequence Name 44-K104418-0022-084-E02C-BH86
GenBank Accession LN505175 [GenBank]
Sequencing primer gene specific primer, BH86: 5'-CACCAAATCGGTAAATCCTAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K104418-0022-084-E02C-BH86
CGGAACGTTTTGAGATCCGACATTTCGACGCTGTGTCTTGTCTGAGCTTAAACGAAGATC
TCGGTTTGCTCTACTCCGGTTCTTGGGACAAGACTCTTAAGGTCTGGAGATTGTCCGATT
CAAAATGTTTAGAATCAATCGAAGCTCACGACGATGCCGTTAACACGGTGGTTTCCGGTT
TCGACGATTTGGTATTTACCGGTTCAGCCGACGGAACGTTGAAAGTATGGAAACGTGAGG
TACAAGGCAAAGAGATGAAGCATGTTTTGGTACAAGTGTTAATGAAACAAGAGAACGCAG
TAACGGCGTTAGCTGTTAATTTAACGGACGCTGTTGTTTACTGTGGTTCGTCTGACGGAA
CCGTTAACTTCTGGGAACGGCAAAAGTATTTGACTCACAAAGGGACGATTCACGGCCACC
GTATGGCGGTTTTGTGTCTCGCCACCGCCGGGAGTTTGCTTTTGAGCGGTGGAGCTGATA
AGAATATTTGCGTTTGGAAAAGAAACGGTGGATAAAACACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCACGTTTATT
   
Sequence Name 60-K104419-0022-084-E02C-8409
GenBank Accession LN510252 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K104419-0022-084-E02C-8409
ACGCAAATATTCTTATCAGCTCCACCGCTCAAAAGCAAACTCCCGGCGGTGGCGAGACAC
AAAACCGCCATACGGTGGCCGTGAATCGTCCCTTTGTGAGTCAAATACTTTTGCCGTTCC
CAGAAGTTAACGGTTCCGTCAGACGAACCACAGTAAACAACAGCGTCCGTTAAATTAACA
GCTAACGCCGTTACTGCGTTCTCTTGTTTCATTAACACTTGTACCAAAACATGCTTCATC
TCTTTGCCTTGTACCTCACGTTTCCATACTTTCAACGTTCCGTCGGCTGAACCGGTAAAT
ACCAAATCGTCGAAACCGGAAACCACCGTGTTAACGGCATCGTCGTGAGCTTCGATTGAT
TCTAAACATTTTGAATCGGACAATCTCCAGACCTTAAGAGTCTTGTCCCAAGAACCGGAG
TAGAGCAAACCGAGATCTTCGTTTAAGCTCAGACAAGACACAGCGTCGAAATGTCGGATC
TTCAAAACGTTTTTCCGGCGACGGACTTCGACGTAGTTTCTAGGATTTACCGATTTGGTG

Last Updated on 06.12.2018 16:11