Sequences from confirmation amplicon


Confirmation sequences for line 085E12 and hit At3g15010 are shown.
Line ID 085E12
Locus At3g15010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5054263
Primers used for PCR PA24 - 8474, show primer details
   
Sequence Name 47-K119373-0022-085-E12C-PA24
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, PA24: 5'-GTATAGAATGCCACCAAGTTCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K119373-0022-085-E12C-PA24
GGTATCCTGGACAGCATCATCAGGGCTGTTGGAACGTCCCCTGTGCCAAGAGTTCCTCAT
GGCGGAATGTACCCCAACGGTCCACCAAACTACTGAAAAGGTGCGGTTGTTCTCCCTTGT
ATATTTTGGGGTTTAAGAGTGTGTTGCATTATCATGATTCATTGCATAGTTATATATTCT
TCCCTACTTTGTGGCATATTGTTTTTGCAGGTTGTGCGTAATTTTCTTGCTAGAAATTCT
CTGCTATTCTTTTCCCTGTGTCTCTTTTAGTCTATTTGCTTGTTAGTACTGTGTTAGCTA
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTACATGT
   
Sequence Name 15-K119372-0022-085-E12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K119372-0022-085-E12C-8409
TTGAATATATCCTGTAGCTAACACANTACTAACAAGCAAATAGACTAAAAGAGACACAGG
GAAAAGAATAGCAGAGAATTTCTAGCAAGAAAATTACGCACAACCTGCAAAAACAATATG
CCACAAAGTAGGGAAGAATATATAACTATGCAATGAATCATGATAATGCAACACACTCTT
AAACCCCAAAATATACAAGGGAGAACAACCGCACCTTTTCAGTAGTTTGGTGGACCGTTG
GGGTACATTCCGCCATGAGGAACTCTTGGCACAGGGGACGTTCCAACAGCCTGATGATGC
TGTCCAGGATACCCAGCAGATGATGAAAGACCGTAATGCCCACTCTCGGGATAACCACCA
CCACCAGGCATCGAACTTGGTGGATTTCTATA

Last Updated on 06.12.2018 16:11