Sequences from confirmation amplicon


Confirmation sequences for line 085H03 and hit At1g09060 are shown.
Line ID 085H03
Locus At1g09060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2924911
Primers used for PCR MK57 - 8474, show primer details
   
Sequence Name 75-K110680-0022-085-H03C-MK57
GenBank Accession LN513260 [GenBank]
Sequencing primer gene specific primer, MK57: 5'-TTCCCTTTAGTGAGTGCTCAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K110680-0022-085-H03C-MK57
TTTTGCATGCAGGTCAATTTTGATGAAACTTGTGATTCAGTGATTAGAATGAATGCTAAT
GAGCAAACTCGATCCGCCAATGGCATTGGCAATGGCAATGGTGAGTCTATTCCCGGGATT
CCAGATGACTTACGGTGCAAGAGATCGGATGGTAAACAGTGGAGATGCACTGCAATGTCC
ATGGCTGATAAGACTGTTTGTGAGAAGCACTACATCCAAGCAAAGAAGCGGGCGGCTAAT
TCTGCTTTCAGGGCGAACCAGAAGAAAGCGAAAAGGCGATCATCGTTAGGCGAAACAGAT
ACGTATTCGGAAGGGAAGATGGATGATTTCGAGTTACCAGTCACCAGCATAACGGTCTTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGNTTATT
   
Sequence Name 67-K110679-0022-085-H03C-8409
GenBank Accession LN511839 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K110679-0022-085-H03C-8409
GCCATTTACAATTGAAGACCGTTATGCTGGTGACTGGTAACTCGAAATCATCCATCTTCC
CTTCCGAATACGTATCTGTTTCGCCTAACGATGATCGCCTTTTCGCTTTCTTCTGGTTCG
CCCTGAAAGCAGAATTAGCCGCCCGCTTCTTTGCTTGGATGTAGTGCTTCTCACAAACAG
TCTTATCAGCCATGGACATTGCAGTGCATCTCCACTGTTTACCATCCGATCTCTTGCACC
GTAAGTCATCTGGAATCCCGGGAATAGACTCACCATTGCCATTGCCAATGCCATTGGCGG
ATCGAGTTTGCTCATTAGCATTCATTCTAATCACTGAATCACAAGTTTCATCAAAATTGA
CCTGCATTGTCAAAACACTAGATTTTCAAACTAAAATCAGAAATTGAGCACTCACTAAAG
GGA

Last Updated on 06.12.2018 16:11