Sequences from confirmation amplicon


Confirmation sequences for line 086A12 and hit At4g00180 are shown.
Line ID 086A12
Locus At4g00180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:72952
Primers used for PCR 17jx - 8474, show primer details
   
Sequence Name 68-K113379-0022-086-A12C-17jx
GenBank Accession LN512051 [GenBank]
Sequencing primer gene specific primer, 17jx: 5'-ATGTTGACAGAGAGGAGATCCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K113379-0022-086-A12C-17jx
GCCTTCGTGCTGCTGCCAAAACGTACCATTTCTTTTTTTAATATATAAAACTAATTTTCT
ATATTGCATTATATCCATAATCATACAATCTCATTTTAGTCTTTATGAATTGAACCTAGC
TAGATTATTGTGGAGCTACATTGATACTCACATTGCTTTAAATTAAAAACATAAAACAAA
ATAGTTACCGTAATAATCCATAATTCATAAGCTTTATCCAGCATACATTAGCTAGGACTA
GCCTTAGGATCTACGATCTAGGGTTTCAGTAAAGTTCCCTAGCAGATATTTTAGTCTGGC
TTTGTATTCGTATATTTTTGACCAAGTTTTTATGTTATGTATAAGATAAATACACATTAA
TGTTAAAAGAGCTAAGTATATGATGTAATATGTATGAAACAGTGGGCTCATTTCCCTCAC
ATACACTTTGGACTCATGGCTGACCATCCTCCCACGAAGAAGGCTAACGTGCGCCAACAG
GTTACTTACTACTACTACACTATATTTATTTATTCAAAACATAATCGAGATATTTTATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 30-K113377-0022-086-A12C-8409
GenBank Accession LN499800 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K113377-0022-086-A12C-8409
GCATTTCATAAAATATCTCGATTATGTTTTGAATAATAAATATAGTGTAGTAGTAGTAAG
TAACCTGTTGGCGCACGTTAGCCTTCTTCGTGGGAGGATGGTCAGCCATGAGTCCAAAGT
GTATGTGAGGGAAATGAGCCCACTGTTTCATACATATTACATCATATACTTAGCTCTTTT
AACATTAATGTGTATTTATCTTATACATAACATAAAAACTTGGTCAAAAATATACGAATA
CAAAGCCAGACTAAAATATCTGCTAGGGAACTTTACTGAAACCCTAGATCGTAGATCCTA
AGGCTAGTCCTAGCTAATGTATGCTGGATAAAGCTTATGAATTATGGATTATTACGGTAA
CTATTTTGTTTTATGTTTTTAATTTAAAGCAATGTGAGTATCAATGTAGCTCCACAATAA
TCTAGCTAGGTTCAATTCATAAAGACTAAAATGAGATTGTATGATTATGGATATAATGCA
ATATAGAAAATTAGTTTTATATATTAAAAAAAGAAATGGTACGTTTTTGGCAGCAGCACT
GAAGGCTTCTCTGTGGCTGATATCAGGGTTGCCTGCCTTTATACGTTGGATCTCCTCTCG
NTCAACA

Last Updated on 06.12.2018 16:11