Sequences from confirmation amplicon


Confirmation sequences for line 086C04 and hit At3g14940 are shown.
Line ID 086C04
Locus At3g14940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5025970
Primers used for PCR 18jx - 8474, show primer details
   
Sequence Name 59-K113393-0022-086-C04C-8409
GenBank Accession LN510052 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K113393-0022-086-C04C-8409
ATTTACATTGAATATTCAATTGAGTATAACTCAGCAGAAAGCTCGTATAACTCTTGAACC
TGTGCACAAGATCATTTTATCAATACATAAATGAATCTAAACAAAAAAGTTCCATCCTTT
ATACATAACTAACACGTAGGTTAAATAATCGGGTAAACTTAAAATCACTAATCGAAACAA
AAAGATGAGATTTTTAGGATCTCTAGCAAATGGGTATCGATTCAATCCAAAGAAGAAGGA
TGTGTACCGTTTCACGGAGATCCTCGCCGTGTAAATCCTGGAGAATGTCGAGAAAGCGAT
CAAGGAGAAGAGCATCGTACTCAACAAGCTTATCGTCTTCACTGACTTTAGCAGGAACGA
GTTGCCGAAGCTGAGCATCAATAGATGCCATCTTCTCTATGTTCCGACCCGCCATTATCG
CCGATCAATCAAATCTTCTTCGCCACCCTCTTACTTCTTCTGTAATCATCAGATCAAAAA
GGAAATGAGTTTTTTTACACAAAAAGGGAAAAAGAAAGGCAAAAT
   
Sequence Name 78-K113394-0022-086-C04C-18jx
GenBank Accession LN513716 [GenBank]
Sequencing primer gene specific primer, 18jx: 5'-GATTTTGCTTTTCTTTTTCCCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K113394-0022-086-C04C-18jx
CTTTTGATCGATGATACGAAGAAGTAGAGGGTGGCGAAGAAGATTTGATTGATCGGCGAT
AATGGCGGGTCGGAACATAGAGAAGATGGCATCTATTGATGCTCAGCTTCGGCAACTCGT
TCCTGCTAAAGTCAGTGAAGACGATAAGCTTGTTGAGTACGATGCTCTTCTCCTTGATCG
CTTTCTCGACATTCTCCAGGATTTACACGGCGAGGATCTCCGTGAAACGGTACACATCCT
TCTTCTTTGGATTGAATCGATACCCATTTGCTAGAGATCCTAAAAATCTCATCTTTTTGT
TTCGATTAGTGATTTTAAGTTTACCCGATTATTTAACCTACGTGTTAGTTATGTATAAAG
GATGGAACTTTTTTGTTTAGATTCATTTATGTATTGATAAAATGATCTTGTGCACAGGTT
CAAGAGTTATACGAGCTTTCTGCTGAGTTATACTCAATTGAATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATT

Last Updated on 06.12.2018 16:11