Sequences from confirmation amplicon


Confirmation sequences for line 086C11 and hit At5g18430 are shown.
Line ID 086C11
Locus At5g18430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6111781
Primers used for PCR 19jx - 8474, show primer details
   
Sequence Name 69-K113549-0022-086-C11C-19jx
GenBank Accession LN512245 [GenBank]
Sequencing primer gene specific primer, 19jx: 5'-TTGTTGGAAGTAATCGAGTTGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K113549-0022-086-C11C-19jx
AATTTATATCTATAATAACCGGAAACTAATATGAAAATTTTCATTTGATGTCAGACTCGG
TTATATATATACTTACGAATTGAAATCCGGTATCATTGAGAATTCCGATACCAGCGGAGG
CGAAATTAGCACCGTTAAGAAGACTCCGGCCCCTTAGCTCTGGACTAAGGTAAGGTAACG
GTGGCTCTTCGTTACCGATTGCCTCACCTAACCAATATTTAACCAGTTTATTATTATGTC
TTTTTGGTAAATAAATAGCTTCTTATGACTAAATATTTGTATACTCATCTGAATCAAGAT
TACTCTAAACCGGTTTATGAGATTAGTAACCAAACGTAACTGATAAGATCTGGAATGTTA
AGACCGTTGGAAAACCGGCCGGTTGGTCTCCGAGTTGGAAAATCGATTCCATACGGTGGA
GAGTCGGCACGTGCAGTTGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCA
   
Sequence Name 04-K113548w-0022-086-C11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K113548w-0022-086-C11C-8409
GACTCTCCCCGTATGGAATCGATTTTCCACTCGGAGACCAACCGGCCGGGTTTTCCAACG
GTCTTAACATTCCAGATCTTATCAGTTACGTTTGGTTACTAATCTCATAAACCGGTTTAG
AGTAATCTTGATTCAGATGAGTATACAAATATTTAGTCATAAGAAGCTATTTATTTACCA
AAAAGACATAATAATAAACTGGTTAAATATTGGTTAGGTGAGGCAATCGGTAACGAAGAG
CCACCGTTACCTTACCTTAGTCCAGAGCTAAGGGGCCGGAGTCTTCTTAACGGTGCTAAT
TTCGCCTCCGCTGGTATCGGAATTCTCAATGATACCGGATTTCAATTCGTAAGTATATAT
ATAACCGAGTCTGACATCAAATGAAAATTTTCATATTAGTTTCCGGTTATTTATTAGATA
TAAATTTTGGCATGCAGATCAACATTATAAGAATGTACCAACAACTCGATTACTTCCAAC
AAA

Last Updated on 06.12.2018 16:11