Sequences from confirmation amplicon


Confirmation sequences for line 086F05 and hit At2g28350 are shown.
Line ID 086F05
Locus At2g28350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12114871
Primers used for PCR CB05 - 3144, show primer details
   
Sequence Name 27-K026772-022-086-F05C-3144
GenBank Accession LN498229 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K026772-022-086-F05C-3144
AGTAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGT
TCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTTCCGCCTTCGGT
TTAAACTATCAGTGTACCACCGGTTGGAGCACTTTCGTTAACCAGAAGAAACTAATCGCC
GGAGACTCAATCGTCTTCCTCCGTTCTGAATCCGGTGACCTCTGCGTCGGAATCCGCCGC
GCTAAACGCGGCGGTCTCGGATCTAACGCAGGATCCGACAATCCTTACCCTGGATTCTCC
GGTTTCCTCCGTGACGACGAGTCAACAACAACAACATCGAAGCTAATGATGATGAAACGC
AACGGAAACAACGACGGAAACGCCGCGGCTACAGGGAGGGTTAGAGTAGA
   
Sequence Name 28-K026772-022-086-F05C-CB05
GenBank Accession LN498695 [GenBank]
Sequencing primer gene specific primer, CB05: 5'-TCCGCTACTGCTTCTACTCTAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K026772-022-086-F05C-CB05
TAGCCGCGGCGTTTCCGTCGTTGTTTCCGTTGCGTTTCATCATCATTAGCTTCGATGTTG
TTGTTGTTGACTCGTCGTCACGGAGGAAACCGGAGATTCCAGGGTAAGGATTGTCGGATC
CTGCGTTAGATCCGAGACCGCCGCGTTTAGCGCGGCGGATTCCGACGCAGAGGTCACCGG
ATTCAGAACGGAGGAAGACGATTGAGTCTCCGGCGATTAGTTTCTTCTGGTTAACGAAAG
TGCTCCAACCGGTGGTACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCC
CCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCG
AAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTT
GCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGG

Last Updated on 06.12.2018 16:11