Sequences from confirmation amplicon


Confirmation sequences for line 087C08 and hit MRC8 are shown.
Line ID 087C08
Locus MRC8 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6174209
Primers used for PCR HN18 - 3144, show primer details
   
Sequence Name 71-K035348-022-087-C08C-HN18
GenBank Accession LN512504 [GenBank]
Sequencing primer gene specific primer, HN18: 5'-CCTCCTTCTTCTTCCTCTTCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K035348-022-087-C08C-HN18
TGATTTCTGTAATAGTTGTGAGCTGAGACCGTAAATTACTAACAGATAAGCGATCACTTG
ATGAAGCAGAGTGACGAGAGCTGGTGCTGTTACTGTTGGTCAGAGGATGATTAAAAGCAT
TCCGAGAATGAGGATGATTATTAACGTTGGTGCTGTGACTAGCACCCATCAAACACCTCC
ACCACCACTTTTAAACTAATTTTAATTTAATTATCATCGATTGACAATAACAATTATTAT
TAGTAAATAATAATGATTTTCACGTCGTCGACGCACGAATCTCTCAGGTATGGCGTGCTT
CTCTCTTTCTAATTCGACTCTCCATTACTATTATTTTTTGTTGGCTGGTTTGGTTCTCTT
GTCCGAAGGATGCGGAAGATAGAAGAAGAAGAAGAAGAAGAAGATGAAAGGTGGGTTTGG
CACGTGCGACTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCC
GGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAA
GGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGC
TTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCA
TCTTTGG
   
Sequence Name 23-K035348-022-087-C08C-3144
GenBank Accession LN496465 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K035348-022-087-C08C-3144
ATCCCCTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGG
ACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTG
AGTCGCACGTGCCAAACCCACCTTTCATCTTCTTCTTCTTCTTCTTCTTCTATCTTCCGC
ATCCTTCGGACAAGAGAACCAAACCAGCCAACAAAAAATAATAGTAATGGAGAGTCGAAT
TAGAAAGAGAGAAGCACGCCATACCTGAGAGATTCGTGCGTCGACGACGTGAAAATCATT
ATTATTTACTAATAATAATTGTTATTGTCAATCGATGATAATTAAATTAAAATTAGTTTA
AAAGTGGTGGTGGAGGTGTTTGATGGGTGCTAGTCACAGCACCAACGTTAATAATCATCC
TCATTCTCGGAATGCTTCTAATCATCCTCTGACCAACAGTAACAGCACCAGCTCTCGTCA
CTCTGCTTCATCAAGTGATCGCTTATCTGTTAGTAATTTACGGTCTCAGCTCACAACTAT
TTACAGAAATCAGGAAGAAGAGGAAGAAGAAGAAGAAGAAGAAGAAGAGGAAGAAAGAAG
GAGG

Last Updated on 06.12.2018 16:11