Sequences from confirmation amplicon


Confirmation sequences for line 088H03 and hit At5g18610 are shown.
Line ID 088H03
Locus At5g18610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6195030
Primers used for PCR DL45 - 8474, show primer details
   
Sequence Name 17-K018474-022-088-H03C-DL45
GenBank Accession LN493664 [GenBank]
Sequencing primer gene specific primer, DL45: 5'-TTTATAGCTTTGGGGTTGTGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K018474-022-088-H03C-DL45
CTCATCACGGGTCCAAAAGCTATTGATAATGCTCGAGCACCCGGAGAGCACAACCTTGTC
GCATGGGTATGTTACTCAACTTTTTCTTATATCACCAAAAGGGAAAAATCACAATTTTTT
TTGCGCTCTCATGTGATATAGTCAATGTTTGGGATCTGAAAACGTCTTTACTGACTTGAA
CTGTTTTGGGGGTTCTCTGTCACTTGTTGGTCTTTGCTTGGCTATACAGGCTAGGCCGTT
GTTCAAAGATCGTAGAAAGTTTCCGAAGATGGCGGATCCATCGCTGCAAGGGCGGTATCC
AATGCGTGGTCTATATCAAGCACTTGCAGTTGCAGCAATGTGTTTACAGGAACAAGCAGC
GACNAGACCACTGATTGGCGACGTGGTGACAGCTCTNACATACTTAGCTTCGCAAACGTT
TGACCCAAACGCACCANGCGGTCAAAACAGTAAAAGTGGGAGTGGGCCACCATTTATCAA
AACAAGGGATGATCGGAGGAGCTTGGGAGATGGGAGTAGCTTGGATAANAAACGTTTTAC
ATGGATCATTCCCAAACGTGTACCANATATTTATCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCANCAATGAGTATGATGGTCAATATGGAGAANAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCANAAATG
   
Sequence Name 18-K018474-022-088-H03C-8409
GenBank Accession LN494109 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K018474-022-088-H03C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATAAATATATGG
TACACGTTTGGGAATGATCCATGTAAAACGTTTTTTATCCAAGCTACTCCCATCTCCCAA
GCTCCTCCGATCATCCCTTGTTCTGATAAATGGTGGCCCACTCCCACTTCTACTGTTTTG
ACCGCTTGGTGCGTTTGGGTCAAACGTTTGCGAAGCTAAGTATGTTAGAGCTGTCACCAC
GTCGCCAATCAGTGGTCTTGTCGCTGCTTGTTCCTGTAAACACATTGCTGCAACTGCAAG
TGCTTGATATAGACCACGCATTGGATACCGCCCTTGCAGCGATGGATCCGCCATCTTCGG
AAACTTTCTACGATCTTTGAACAACGGCCTAGCCTGTATAGCCAAGCAAAGACCAACAAG
TGACAGAGAACCCCCAAAACAGTTCAAGTCAGTAAAGACGTTTTCAGATCCCAAACATTG
ACTATATCACATGAGAGCGCAAAAAAAATTGTGATTTTTCCCTTTTGGTGATATAAGAAA
AAGTTGAGTAACATACCCATGCGACAAGGTTGTGCTCTCCGGGTGCTCGAGCATTATCAA
TAGCTTTTCGACCCGTGATGAGCTCGAGAAACACAACCC

Last Updated on 06.12.2018 16:11