Sequences from confirmation amplicon


Confirmation sequences for line 089F02 and hit At3g02570 are shown.
Line ID 089F02
Locus At3g02570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:543539
Primers used for PCR 01ht - 8474, show primer details
   
Sequence Name 30-K112586-0022-089-F02C-01ht
GenBank Accession LN499788 [GenBank]
Sequencing primer gene specific primer, 01ht: 5'-TCAACTATGTGAAGCTCAATCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K112586-0022-089-F02C-01ht
GCATACTTATTCGGTGAGTGCCTTGAGGTCATGGCGACTTCAGACAATGTAGTACGAGCT
GGCCTCACTTCCAAGCCTCTGGATATTCAAACACTTTGTTCAATGCTCTCTTATAAACTG
GTAAAGATCTAAACCAAACTCAAAGTGTACTCCAAGACTTACAAAAGATTGGTTACTAAG
GTCTTATTTCTGACTTTCAGGGATATCCGGAAATCCTGAAGGGAACAAGGATTCGACCAT
ACATAACAAGATACCTGCCGCCTTTCGAAGAGTTTGAAGTTGATCTGTGTGATCTTCCCA
GTGGAGCCTCGACGGTGTTTCCCTCTGTTCCAGGCCCTTCTCTTCTGCTTGTACTCCAAG
GTGAAGGAAGAATGTCTACAGAAGCATCAGCCGATGGGATTTCAATGGGAGATGTTTTAT
TTGTTCCTGCAGATACCGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 04-K112585-0022-089-F02C-8409
GenBank Accession LN487985 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K112585-0022-089-F02C-8409
GATATCGGTATCTGCAGGAACAATAAAACATCTCCCATTGAAATCCCATCGGCTGATGCT
TCTGTAGACATTCTTCCTTCACCTTGGAGTACAAGCAGAAGAGAAGGGCCTGGAACAGAG
GGAAACACCGTCGAGGCTCCACTGGGAAGATCACACAGATCAACTTCAAACTCTTCGAAA
GGCGGCAGGTATCTTGTTATGTATGGTCGAATCCTTGTTCCCTTCAGGATTTCCGGATAT
CCCTGAAAGTCAGAAATAAGACCTTAGTAACCAATCTTTTGTAAGTCTTGGAGTACACTT
TGAGTTTGGTTTAGATCTTTACCAGTTTATAAGAGAGCATTGAACAAAGTGTTTGAATAT
CCAGAGGCTTGGAAGTGAGGCCAGCTCGTACTACATTGTCTGAAGTCGCCATGACCTCAA
GGCACTCACCGAATAAGTATGCGTGGGGCTCGTTTGCACCAAGATACAAGGCTTCGCCAG
GATTGAGCTTCCANTAGTTGA

Last Updated on 06.12.2018 16:11