Sequences from confirmation amplicon


Confirmation sequences for line 089F04 and hit At2g42500 are shown.
Line ID 089F04
Locus At2g42500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17698846
Primers used for PCR NB06 - 8474, show primer details
   
Sequence Name 18-K101268-0022-089-F04C-8409
GenBank Accession LN494232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K101268-0022-089-F04C-8409
ATTTACATTGAATATATCCTGGAAACATAATGATTTGATGATTCCCAAAAATTTGTGTAG
AAAATACCAACGATTTAGTCAGATAAGGCATACATGAAATCTTCCATATATAACAGATCT
CCATTCTTTGCATTTTTCAGAATACTAACGTCTTGCAATTGATGTGACACTAGCAAAAGA
AATACGACAGCACAGTACAAAAGTACTACTTTGGTTCCAAATAATACTGCCAGCACAAGA
CAAAGAACTGATCATCTATATTAATTGACTACCGGACGAGGATAGACTTATATGTGAACC
CCCACTAACAAAAAAGGAAGAAAAATGTGAGACATGTATGATAGGTACTAACCAAGGCTG
TCAGAGGAAAATAGTCGAAGAGGTCTGTAAAGATTTTCCAAACATTTGCGTTGCCGTACC
TAAAAAGGAAAACATGGTTTGTTAAATTTATTGCAAAATCTATATCCCAAATAAGCTGCA
AACTAGGATGTGTAAGATTATACAACATAGAACAATACTATAAGCAAGTAATCATAAGCC
CAAAGGATTAACAGAAACAGGAAGTAAAGAAAGAAAAGACAATTTTGGACAGGCTACCAT
ACACA
   
Sequence Name 18-K101274-0022-089-F04C-NB06
GenBank Accession LN494233 [GenBank]
Sequencing primer gene specific primer, NB06: 5'-GCATTGTATGTGTGTATGGTAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K101274-0022-089-F04C-NB06
TTTCGTAATCCTTTGGGCTTATGATTACTTGCTTATAGTATTGTTCTATGTTGTATAATC
TTACACATCCTAGTTTGCAGCTTATTTGGGATATAGATTTTGCAATAAATTTAACAAACC
ATGTTTTCCTTTTTAGGTACGGCAACGCAAATGTTTGGAAAATCTTTACAGACCTCTTCG
ACTATTTTCCTCTGACAGCCTTGGTTAGTACCTATCATACATGTCTCACATTTTTCTTCC
TTTTTTGTTAGTGGGGGTTCACATATAAGTCTATCCTCGTCCGGTAGTCAATTAATATAG
ATGATCAGTTCTTTGTCTTGTGCTGGCAGTATTATTTGGAACCAAAGTAGTACTTTTGTA
CTGTGCTGTCGTATTTCTTTTGCTAGTGTCACATCAATTGCAAGACGTTAGTATTCTGAA
AAATGCAAAGAATGGAGATCTGTTATATATGGAAGATTTCATGTATGCCTTATCTGACTA
AATCGTTGGTATTTTCTACACAAATTTTTGGGAATCATCAAATCATTATGTTTCCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG
AT

Last Updated on 06.12.2018 16:11