Sequences from confirmation amplicon


Confirmation sequences for line 090A05 and hit At2g28940 are shown.
Line ID 090A05
Locus At2g28940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12427343
Primers used for PCR 04fu - 8474, show primer details
   
Sequence Name 32-K111563-0022-090-A05C-04fu
GenBank Accession LN500637 [GenBank]
Sequencing primer gene specific primer, 04fu: 5'-ATTGTGCTGATGATGATGAAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K111563-0022-090-A05C-04fu
ACTTTGTGTATAAGAGCTTGGAGGATCATCTCGTAGGCCGAGTTGTGTCGGTATCTCTAC
CGTGGATGATGAGGTTGAAGATTGCGCAAGATGCTGCTCAAGGCTTAGCTTATCTTCATG
AAGAAATGGATTTTCAGGTTAAGCATTTCATTGCCATTTATATGATTTAAGAGTGTTTTG
TGTTTTGGTTTTGAATTTGCTTCTGTTGATCTTTTAGTTGATATTTCGAGATTTCAAGTC
TTCAAACATCTTGCTTGATGAGAGATTCGGTGCAAAGCTCTCGGATTTTGGATTAGCTAG
GCAAGGACCTCCTGAAGGACTTGGTCATGTCTCAACTTCAGTATGTCTCTATTGGAGCAC
TCTTTAACATAACATCTCTNGAAGTCTTTAACTTTGTCTTGTATGGAAGGTTGTAGGTAC
GGTTGGTTACGCAGCTCCTGAGTATGTACAAACCGGGAAATTGACGGCAAAGAGTGATGT
ATGTAGATTTCCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACGGT
   
Sequence Name 16-K111556w-0022-090-A05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K111556w-0022-090-A05C-8409
CATTTCATTGAATTATCCTGGGAAATCACATACATCACTCTTTGCCGTCAATTTCCCGGT
TTGTACATACTCAGGAGCTGCGTAACCAACCGTACCTACAACCTTCCATACAAGACAAAG
TTAAAGACTTCAAGAGATGTTATGTTAAAGAGTGCTCCAATAGAGACATACTGAAGTTGA
GACATGACCAAGTCCTTCAGGAGGTCCTTGCCTAGCTAATCCAAAATCCGAGAGCTTTGC
ACCGAATCTCTCATCAAGCAAGATGTTTGAAGACTTGAAATCTCGAAATATCAACTAAAA
GATCAACAGAAGCAAATTCAAAACCAAAACACAAAACACTCTTAAATCATATAAATGGCA
ATGAAATGCTTAACCTGAAAATCCATTTCTTCATGAAGATAAGCTAAGCCTTGAGCAGCA
TCTTGCGCAATCTTCAACCTCATCATCCACGGTAGAGATACCGACACAACTCGGCCTACG
AGATGATCCTCCAAGCTCTTATTACACATAAGTTCATAGACTAGAAGCCTTTGCATCCCT
CTNTCATCATCACAGGCACAA

Last Updated on 06.12.2018 16:11