Sequences from confirmation amplicon


Confirmation sequences for line 090F10 and hit At1g52540 are shown.
Line ID 090F10
Locus At1g52540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19571299
Primers used for PCR 05hg - 8474, show primer details
   
Sequence Name 40-K112551-0022-090-F10C-05hg
GenBank Accession LN503756 [GenBank]
Sequencing primer gene specific primer, 05hg: 5'-CAGATTCGATACATTCTGGTGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K112551-0022-090-F10C-05hg
CTCATCATCTGGCATCAGCTTATCGTATCCGAAATCTGTAACCCGAGCTTCAAACTCAGA
ATCTAGCAGCACATTGCTTGCTCTCACATCTCCATGGACTATTCTAGGTGTTGCAAAATG
ATGCAAGTAGCTGTAAAAAAAATGGAAACGAATAAATCAGTCAACAGTAGATGTTGCTAA
TTTATATGAACTTTTCTTGATCCCGAAAACTTACGCAATCGCCTGAGCAGAAGATACAGC
AATATTCATCCGCCTAGTCCAATCAAGAAGCGACTCAGATGAATGTTGACCATGAAGATG
AGAGACCAAGCTCAAATTTGGCATGTAATCATATACAATGAGTCGTTCTTGTCCTTCTGC
ACAGTAACCTCGTACACTCAATAGATTCTTGTGACGGATACGAGCAAGAATCTCGACTTC
TACAGCAAAATCTATCTCTTCTCTGCTACTCCATGCCTTCAATCTCTTGACTGCAATCTA
CAGACAAAAACAAAAGCTCATTACAGTATGAAGATCAAAGAGACCACATCTACATGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCC
   
Sequence Name 16-K112550-0022-090-F10C-8409
GenBank Accession LN493493 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K112550-0022-090-F10C-8409
TTGAATATATCATGTAGATGTGGTCTCTTTGATCTTCATACTGTAATGAGCTTTTGTTTT
TGTCTGTAGATTGCAGTCAAGAGATTGAAGGCATGGAGTAGCAGAGAAGAGATAGATTTT
GCTGTAGAAGTCGAGATTCTTGCTCGTATCCGTCACAAGAATCTATTGAGTGTACGAGGT
TACTGTGCAGAAGGACAAGAACGACTCATTGTATATGATTACATGCCAAATTTGAGCTTG
GTCTCTCATCTTCATGGTCAACATTCATCTGAGTCGCTTCTTGATTGGACTAGGCGGATG
AATATTGCTGTATCTTCTGCTCAGGCGATTGCGTAAGTTTTCGGGATCAAGAAAAGTTCA
TATAAATTAGCAACATCTACTGTTGACTGATTTATTCGTTTCCATTTTTTTTACAGCTAC
TTGCATCATTTTGCAACACCTAGAATAGTCCATGGAGATGTGAGAGCAAGCAATGTGCTG
CTAGATTCTGAGTTTGAAGCTCGGGTTACAGATTTCGGATACGATAAGCTGATGCCAGAT
GATGGAGCTAACAAAAGCACCAAGGGTAATAACATTGGGTATCTCTCACCAG

Last Updated on 06.12.2018 16:11