Sequences from confirmation amplicon


Confirmation sequences for line 091B09 and hit At5g09410 are shown.
Line ID 091B09
Locus At5g09410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2923138
Primers used for PCR 06ke - 8474, show primer details
   
Sequence Name 63-K113393-0022-091-B09C-8409
GenBank Accession LN511030 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K113393-0022-091-B09C-8409
TCGGATGAGCATTTATAATTTTATGTAAATGATGGAGTAATATCACAGACAGGACGAGCT
ATACCTTAACCTCCAAGTAGTGCACAAAAACGATATGCATCAAATGTCTGTAACAATACA
GCATAAAACAAAGTATATAAGAACAAAAGTGAAAACAGACTCAAAAAGCAAAGTCAACTC
GTACAAGGGAGACACTAAACTATTTAGGAGACACTAAAGAAAATTCCAATGTTCACTATT
TCCATGTTCCACACCTAACCCACGAAGGCCTCAGATATCCAAAGGAAAACAAAGACAAAG
TAGCGACTAAAACCCAATGAGATGAGGCTTTTCTATAATAGTACCTTCAAAGTTATGATC
ATAAACACTATTTTTTTCCGTCCAAGCTAGAAGGTCAGTATACACAGAGAACAACAAAAC
ACTGAAATATGCACAAACTCAGACTCACGNTTCAAG
   
Sequence Name 82-K113394-0022-091-B09C-06ke
GenBank Accession LN514215 [GenBank]
Sequencing primer gene specific primer, 06ke: 5'-GCTTGAACAGTGAGTCTGAGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K113394-0022-091-B09C-06ke
ACCTTCTGCTTGGACGGAAAAAATAGTGTTTATGATCATAACTTTGAAGGTACTATTATA
GAAAAGCCTCATCTCATTGGGTTTTAGTCGCTACTTTGTCTTTGTTTTCCTTTGGATATC
TGAGGCCTTCGTGGGTTAGGTGTGGAACATGGAAATAGTGAACATTGGAATTTTCTTTAG
TGTCTCCTAAATAGTTTAGTGTCTCCCTTGTACGAGTTGACTTTGCTTTTTGAGTCTGTT
TTCACTTTTGTTCTTATATACTTTGTTTTATGCTGTATTGTTACAGACATTTGATGCATA
TCGTTTTTGTGCACTACTTGGAGGTTAAGGTATAGCTCGTCCTGTCTGTGATATTACTCC
ATCATTTAACATTAAAATTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGNTTTATT

Last Updated on 06.12.2018 16:11