Sequences from confirmation amplicon


Confirmation sequences for line 091H02 and hit At4g35160 are shown.
Line ID 091H02
Locus At4g35160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16732492
Primers used for PCR EP02 - 8474, show primer details
   
Sequence Name 25-K103231-0022-091-H02C-8409
GenBank Accession LN497397 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K103231-0022-091-H02C-8409
TTCGGACTGAGCCTTTACATTGAATATCATCTACAGGCTACGTTAATACGCCACTCTCTC
GCCGTTTGATGATCACAAGACGTGATGGAAAATCGCTGGCTCCTTTTGTTCTCTTCGAAA
CAACTCCCGAGATGCTCGCTCCATGGTTGAGACTTAGCTCAGTCGTTTCTTCGCCGGTCA
ACGGTTCAACTCCACCACCGTTTGATGCAGTGCACGGTAAGGACGTGTGGTCGTTCGCGC
AGGATAATCCCTTCCTCAGCGATATGATCAATGAGGCCATGGCTTGTGATGCAAGGCGCG
TGGTGCCACGTGTAGCCGGAGCTTGTCACGGCTTGTTTGATGGCGTGACTACGATGGTTG
ACGTAGGAGGTGGTACGGGAGAGACGATGGGGATGCTTGTGAAGGAGTTTCCTTGGATCA
AAGGATTTAACTTTGATCTTCCTCATGTCATTGAAGTTGCTGAAGTCTTGGACGGTGTTG
AGAATGTTGAGGGCGATATGTTTGATTCTATTCCGGCTTGCGACGCCATTTTCATCAAGG
TTAAATTCATTTCCATACTCTTATTTTCTTTTGATTAATAATAATATTCACTATGATTAC
TTTTTTTCTTCTTCTTACAACTTCCATTTAGTGTTCAAAACAAATTTTACATAAATGAAA
AGATTAAAAAAATATCACTAAAAATCTAGTTTTATTCTTTCTTACAACTTTAATTCGTGT
TCAAAATCAAATTTCACATAAAAGACAAGAAAAAAATGAATTAATGTTATAAACTAACTT
TTTTTTCTTAATTCTAATTAAGTTGATTTGTTGCCGT
   
Sequence Name 73-K103232-0022-091-H02C-EP02
GenBank Accession LN512876 [GenBank]
Sequencing primer gene specific primer, EP02: 5'-AAACAAAAATGTTTTTACGGCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K103232-0022-091-H02C-EP02
AAGTTGTTTATACATTAATTCATTTTTTTCTTGTCTTTTATGTGACATTTGATTTTGAAC
ACGAATTAAAGTTGTAAGAAAGAATAAAACTAGATTTTTAGTGATATTTTTTTAATCTTT
TCATTTATGTAAAATTTGTTTTGAACACTAAATGGAAGTTGTAAGAAGAAGAAAAAAAGT
AATCATAGTGAATATTATTATTAATCAAAAGAAAATAAGAGTATGGAAATGAATTTAACC
TTGATGAAAATGGCGTCGCAAGCCGGAATAGAATCAAACATATCGGCCTCAACATTCTCA
ACACCGTCCAAGACTTCAGCAACTTCAATGACATGAGGAAGATCAAAGTTAAATCCTTTG
ATCCAAGGAAACTCCTTCACAAGCATCCCCATCGTCTCTCCCGTACCACCTCCTACATCA
ACCATCGTAGTCACGCCATCAAACAAGCCGTGACAAGCTCCGGCTACACGTGGCACCACG
CGCCTTGCATCACAAGCCATGGCCTCATTGATCATATCGCTGAGGAAGGGATTATCCTGC
GCGAACGACCACACGTCCTTACCGTGCACTGCATCAAACGGTGGTGGAGTTGAACCGTTG
ACCGGCGAAGAAACGACTGAGCTAAGTCTCAACCATGGAGCGAGCATCTCGGGAGTTGTT
TCGAAGAGAACAAAAGGAGCCAGCGATTTTCCATCACGTCTTGTGATCATCAAACGGCGA
GAGAGTGGCGTATTAACGTAGCCAGTAGATGTATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGAACAATTACGATCCGTCGTATTTATGGGCGAAAGCAAT

Last Updated on 06.12.2018 16:11