Sequences from confirmation amplicon


Confirmation sequences for line 092A09 and hit At1g70120 are shown.
Line ID 092A09
Locus At1g70120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26409459
Primers used for PCR 03ui - 8474, show primer details
   
Sequence Name 38-K117118-0022-092-A09C-03ui
GenBank Accession LN503127 [GenBank]
Sequencing primer gene specific primer, 03ui: 5'-TTTATTGATAAGGGTCCCCAGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K117118-0022-092-A09C-03ui
TCAGCTTCATTGTTTTACAGACTATTAATCTTGTTACCTCATCTGCACAAAGGTTATATA
TAATTTATTCGATTATAAATTAATCTTATAGTATTTTATTTAAAACTGTTTATAATTCTG
ACAAAAAATTGCTTGCATATAAAATAGGTATATCGATCTCAAATCTGGCACAGCTCAACA
AATTAGGGTTTCAGTAGAAGAAGATATTGACGGTTTGATTAGGAAAAGGATTATCAAAGA
TATCAAAGAAAAGAGAGAAGTGAAGTTCGGATCGCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATAATA
A
   
Sequence Name 16-K117117-0022-092-A09C-8409
GenBank Accession LN493601 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117117-0022-092-A09C-8409
TCCTGCGATCCGAACTTCACTTCTCTCTTTTCTTTGATATCTTTGATAATCCTTTTCCTA
ATCAAACCGTCAATATCTTCTTCTACTGAAACCCTAATTTGTTGAGCTGTGCCAGATTTG
AGATCGATATACCTATTTTATATGCAAGCAATTTTTTGTCAGAATTATAAACAGTTTTAA
ATAAAATACTATAAGATTAATTTATAATCGAATAAATTATATATAACCTTTGTGCAGATG
AGGTAACAAGATTAATAGTCTTGTAAAACAATGAAGCTTGAATGTCTCCTTGGAGACATA
TATAGGCATTAGGAAGGTTACTGGGGACCCTTATCAATAAA

Last Updated on 06.12.2018 16:11