Sequences from confirmation amplicon


Confirmation sequences for line 094C03 and hit At1g64150 are shown.
Line ID 094C03
Locus At1g64150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23811497
Primers used for PCR RK31 - 8474, show primer details
   
Sequence Name 27-K019216-022-094-C03C-RK31
GenBank Accession LN498177 [GenBank]
Sequencing primer gene specific primer, RK31: 5'-ACTGAACCAAAACCAAAACAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K019216-022-094-C03C-RK31
TAACAAAAGGGTTACAGCTACATTTGGTTTCACTTGGTAGACATCATATACTTAGGAAGA
TATACTTGCTAGACATAAGAAAGGTCCAAACCAAGCACCCGTGGTTAGTACGACTCTGCT
AAGAAAGGTGAATAAAGAGATTTACCAGAAGACAAACTGCTGCAATATCATCTATAGGCA
AATCGGTTCCACCAAACCTGTGTTCTCAAGCATTGACGTTCAGCATCATCATGGAACTTA
AACCTGCTTAATAATTTTTGCCAGAATACTCAAACCTGAATGGAAGAACTTCATCGACAT
AGTGGAAAGTGCGTCCAAGCACTACAGATATTATCGTCATTATCCTGCAAAATTACCAAG
TTTTATAACGTTGCTCTACAGATTTCAAAGTAACATTTGGTTTCAGACAAGATTCACGGA
CCATAATAACGCCTCTGTTTGAGAAAGAAAGCATACCCTAGAGCGCCAAAAGTCCCCACG
AATACGGTAGCAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCT
   
Sequence Name 26-K019216-022-094-C03C-8409
GenBank Accession LN497712 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K019216-022-094-C03C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCTGCTACCGTATTCG
TGGGGACTTTTGGCGCTCTAGGGTATGCTTTCTTTCTCAAACAGAGGCGTTATTATGGTC
CGTGAATCTTGTCTGAAACCAAATGTTACTTTGAAATCTGTAGAGCAACGTTATAAAACT
TGGTAATTTTGCAGGATAATGACGATAATATCTGTAGTGCTTGGACGCACTTTCCACTAT
GTCGATGAAGTTCTTCCATTCAGGTTTGAGTATTCTGGCAAAAATTATTAAGCAGGTTTA
AGTTCCATGATGATGCTGAACGTCAATGCTTGAGAACACAGGTTTGGTGGAACCGATTTG
CCTATAGATGATATTGCAGCAGTTTGTCTTCTGGTAAATCTCTTTATTCACCTTTCTTAG
CAGAGTCGTACTAACCACGGGTGCTTGGTTTGGACCTTTCTTATGTCTAGCAAGTATATC
TTCCTAAGTATATGATGTCTACCAAGTGAAACCAAATGTAGCTGTAACCCTTTTGTTATG
AGTTTTGTTTTG

Last Updated on 06.12.2018 16:11