Sequences from confirmation amplicon


Confirmation sequences for line 095A10 and hit At3g59550 are shown.
Line ID 095A10
Locus At3g59550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21998436
Primers used for PCR CM13 - 8474, show primer details
   
Sequence Name 03-K026963-022-095-A10C-8409
GenBank Accession LN487334 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K026963-022-095-A10C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTATGCCTACTCACAGGT
TCTGTTGATTGGCTGCTCAAAGCAAAAAAATATGTAACAACATTATTAAAGAAGATCAAC
TAAACAGATAAGGAAGATTAAGAGCAAGGTGTAGTAAAGGAAATCAAAACTCTATGAAGA
GCCTCACTCGACATCCATCGGGATAGATTCTGAGATGATGTCCTGTAAGAAAGGCATACA
AACTTTCATTCAATAACAAGTGCTCGGAGACATGGGAAACAAGAAGTCAGAATCAACTTC
AAAGTCATAAGAAGCAAGCAGTAATCTCACCTCGTCGAATGTTACAGCAACATAAGGATC
AATGCCAGTAGGAATTTGATCTGAAAAATAAGCATCAACATCATAAACACCTCAAAATCA
TGGTATGCTGCATCTAGAACCAAGGAAAAAAAACACAGACAAAAGGTACCTGTGAGAGTG
ATATCTTCCTCACTCCGAGTATGATTATCGAATTCCCTAGAATTCACAAGAAAAGAATGT
TAAAGATGAGTCAGGCTTTAGAACAGATAAAAACTCAAATAAAAGCAAGAAAGAAGAATA
CATGTCGAGAGTGTCATCTTCCAAGTC
   
Sequence Name 07-K026963-022-095-A10C-CM13
GenBank Accession LN489115 [GenBank]
Sequencing primer gene specific primer, CM13: 5'-TCTGTTACTTTGCCTCAAGCACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K026963-022-095-A10C-CM13
CTGGACGAGTTCGACTTGGAAGATGACACTCTCGACATGTATTCTTCTTTCTTGCTTTTA
TTTGAGTTTTTATCTGTTCTAAAGCCTGACTCATCTTTAACATTCTTTTCTTGTGAATTC
TAGGGAATTCGATAATCATACTCGGAGTGAGGAAGATATCACTCTCACAGGTACCTTTTG
TCTGTGTTTTTTTTCCTTGGTTCTAGATGCAGCATACCATGATTTTGAGGTGTTTATGAT
GTTGATGCTTATTTTTCAGATCAAATTCCTACTGGCATTGATCCTTATGTTGCTGTAACA
TTCGACGAGGTGAGATTACTGCTTGCTTCTTATGACTTTGAAGTTGATTCTGACTTCTTG
TTTCCCATGTCTCCGAGCACTTGTTATTGAATGAAAGTTTGTATGCCTTTCTTACAGGAC
ATCATCTCAGAATCTATCCCGATGGATGTCGAGTGAGGCTCTTCATAGAGTTTTGATTTC
CTTTACTACACCTTGCTCTTAATCTTCCTTATCTGTTTAGTTGATCTTCTTTAATAATGT
TGTTACATATTTTTTTGCTTTGAGCAGCCAATCAACAGAACCTGTGAGTAGGCATACAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAA

Last Updated on 06.12.2018 16:11