Sequences from confirmation amplicon


Confirmation sequences for line 095B03 and hit At1g47980 are shown.
Line ID 095B03
Locus At1g47980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17693317
Primers used for PCR 74cs - 3144, show primer details
   
Sequence Name 28-K109029-0022-095-B03C-74cs
GenBank Accession LN498882 [GenBank]
Sequencing primer gene specific primer, 74cs: 5'-GGAGTTTCCATTGAATTTGGAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K109029-0022-095-B03C-74cs
GTTGGGCTTGGTCTTGATAAAGTCGCACCAAATCTAACAATGGGAGGGCCAAGTCCTATT
GGAGCTCAGAAAGCCAATCTCGATCCTTTGACAAGAGATATTATTTTGCAGTTTGCTTGG
CAAGAGGTTGGCCACTTGAGGTAAAATGTTTCTTTTAATTCATATATAGTATGTATAGTT
GAGATCGAATTGTGATTATATTACCTGTCTAGGAGGTATGGATTAGTGTCTTTAGAAAAA
TATAAGAAATTTGTTTTCCTTATTATTTAGTTAGACTAGGAAACTTTTTTTTTTTTTTTT
TTTTTTTTGTGTTTTTTCCTGGAAAAAAAAATTTTTTCCCCAAAAAAAATGTTTTTAAAA
TTTTTTTTTAACCCAAAAAAATTGAAGGAATTTTTTCCCTTTTTCCCCCCAAAATTTAAA
CGGGAAAAAACCCCCCCAAAATTTTTTTTCAAACCCGGGATTTTTAAACCAAAGGGGGGA
AAAAAAAATTTTAACCCCGGGGACCCCGCCCCCCCCAAAAAAAAAAAATTTCCTTTTTTA
AAAAAAGGGTTTTCCAAAAAAAAAGGGGATTTGGCCCCCCCCCCTCCCCCCGGGGAAATT
CCAACCCCCCCAAAAAAAA
   
Sequence Name 12-K109028-0022-095-B03C-3144
GenBank Accession LN491616 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K109028-0022-095-B03C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAACTGATAT
TTGGATGGATTTTTGACAGTTTAAATTTTGCGATGCAAATGTGACAAATATCCTTCAAGT
TTTTCGGTTATAAATAATATATTAAAGACATTTCGTCTGAGAGACAAATTACTTCTCCAA
GACAACAACAACAAAAAAAAAAAAAAAAAAAAAAATTTTCCCTCCCCAACCAAAAAAAAG
GGAAAAAAATTTTTTTTTTTTTTTTTAAAAAACCTAACCCCTCCCCCCCAAAAGGGGAAA
AAAAACCCAATTCCTTCCCCACTTTCCCTCCTTTTTTTAAATTAAAAAAAACTTTTTCCC
CCAGGGGGCCCACCTTTTGCCAAAAAAAAGGAAAAAAAAATTTTTTTTTAAAAAGAACAA
AATTGGTTTTTTAAACCCCAAAAAAAATTGGCCCCCCCCTTGGAAAATTTGGGGGCACTT
TTTAAAAAACAACCCCTAAAGCTCCAAAAAAAAAAAACTCCCCTTCCGGGGCCCCCCAT

Last Updated on 06.12.2018 16:11