Sequences from confirmation amplicon


Confirmation sequences for line 095C01 and hit At1g27770 are shown.
Line ID 095C01
Locus At1g27770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9674600
Primers used for PCR YI02 - 8474, show primer details
   
Sequence Name 23-K027344-022-095-C01C-8409
GenBank Accession LN496417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K027344-022-095-C01C-8409
GCCATTTACAATTGACTGGAGAAAGTGAACCGGTTAGTGTCAGTGTGGAACACCCGTTTC
TTCTCTCTGGTACCAAGGTTCAGGATGGATCTTGCAAAATGTTGGTGACTACAGTTGGAA
TGAGGACTCAATGGGGTAAACTGATGGCTACACTAAGTGAAGGCGGTGATGATGAGACAC
CGTTGCAAGTAAAACTTAATGGTGTGGCAACCATCATTGGTAAAATAGGTCTATTTTTTG
CTGTCATTACGTTTGCTGTTTTGGTTCAAGGATTGGCTAATCAGAAGCGCCTAGATAATT
CTCACTGGATCTGGACTGCTGATGAATTGATGGCGATGCTAGAATATTTTGCTGTTGC
   
Sequence Name 24-K027344-022-095-C01C-YI02
GenBank Accession LN496854 [GenBank]
Sequencing primer gene specific primer, YI02: 5'-CAATTGTTACAGCAACAGCAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K027344-022-095-C01C-YC01
GCAGTCAGATCCAGTGAGAATTATCTAGGCGCTTCTGATTAGCCAATCCTTGAACCAAAA
CAGCAAACGTAATGACAGCAAAAAATAGACCTATTTTACCAATGATGGTTGCCACACCAT
TAAGTTTTACTTGCAACGGTGTCTCATCATCACCGCCTTCACTTAGTGTAGCCATCAGTT
TACCCCATTGAGTCCTCATTCCAACTGTAGTCACCAACATTTTGCAAGATCCATCCTGAA
CCTTGGTACCAGAGAGAAGAAACGGGTGTTCCACACTGACACTAACCGGTTCACTTTCTC
CAGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTC

Last Updated on 06.12.2018 16:11