Sequences from confirmation amplicon


Confirmation sequences for line 096D09 and hit At3g12160 are shown.
Line ID 096D09
Locus At3g12160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3880016
Primers used for PCR CV20 - 8474, show primer details
   
Sequence Name 07-K030752-022-096-D09C-8409
GenBank Accession LN489147 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K030752-022-096-D09C-8409
ATGAGCCATTTACAATTAAAATGGGCAACATGAATATGTGATGTTTGATCATCACTTTGT
TTAAGGCTCTTACGTTTATCAAAGTGTTTCTTTGGCAGATATAGGGCGGTGACAAGTGCA
TACTACCGTGGAGCAGTTGGGGCAATGTTGGTCTACGACATGACCAAACGTCAATCATTC
GATCACATGGCTAAATGGCTGGAAGAGTTACGAGGACATGCGGACAAGAACATAGTGATT
ATGCTAATTGGGAACAAATGTGATCTTGGAAGCCTAAGAGCAGTGCCAACAGAAGACGCA
CAAGAGTTTGCACAAAGAGAAAACTTGTTTTTCATGGAAACCTCTGCTCTAGAAGCTACC
AATGTTGAAACAGCATTTTTGACCATCTTAACAGAGATCTACCGAATAATCAGCAAGAAA
TCACTAACGGCTGATGATGATGACGCCGATGGTAATTCAAGTCTCTTGAAAGGAACTAGA
ATCATCATCCCGAGCGAGCAAGAGAGTGGGAAAAGAGGTGGATGTTGCGGCAAATCGTAA
TTCTACTAGCGAAAGAGAGAAAGATTGTATTTTTTGGTTGTTCTTGTTGTTTTTTCTTTG
TCCCGCTTTATATATTTTGTTATTCGTGTTAGGCCATTTGTTCTTGAAACGAAACAATAT
GCCAGAGGTTT
   
Sequence Name 46-K030752-022-096-D09C-CV20
GenBank Accession LN505763 [GenBank]
Sequencing primer gene specific primer, CV20: 5'-GTAAACCTTGGCATATTGTTTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K030752-022-096-D09C-CV20
GGCTACCGAATACAAATATATAAAGCGGGACAAAGAAAAAACAACAAGAACAACCAAAAA
ATACAATCTTTCTCTCTTTCGCTAGTAGAATTACGATTTGCCGCAACATCCACCCTCTTT
TCCCACTCTCTTGCTCGCTCGGGATGATGATTCTAGTTCCTTTCAAGAGACTTGAATTAC
CATCGGCGTCATCATCATCAGCCGTTAGTGATTTCTTGCTGATTATTCGGTAGATCTCTG
TTAAGATGGTCAAAAATGCTGTTTCAACATTGGTAGCTTCTAGAGCAGAGGTTTCCATGA
AAAACAAGTTTTCTCTTTGTGCAAACTCTTGTGCGTCTTCTGTTGGCACTGCTCTTAGGC
TTCCAAGATCACATTTGTTCCCAATTAGCATAATCACTATGTTCTTGTCCGCATGTCCTC
GTAACTCTTCCAGCCATTTAGCCATGTGATCGAATGATTGACGTTTGGTCATGTCGTAGA
CCAACATTGCCCCAACTGCTCCACGGTAGTATGCACTTGTCACCGCCCTATATCTGCCAA
AGAAACACTTTGATAAACGTAAGAGCCTTAAACAAAGTGATGATCAAACATCACATATTC
ATGTTGCCCATTTTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11