Sequences from confirmation amplicon


Confirmation sequences for line 097A03 and hit At1g10300 are shown.
Line ID 097A03
Locus At1g10300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3378198
Primers used for PCR BH75 - 8474, show primer details
   
Sequence Name 09-K104381-0022-097-A03C-BH75
GenBank Accession LN490161 [GenBank]
Sequencing primer gene specific primer, BH75: 5'-AGGACATCCACAGATCAAGAGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K104381-0022-097-A03C-BH75
AGCCTTGCATGTGTTGCCTAACCTGTTCAGATAAGCCAAACTAGGACCAATACCTTTCAT
AACAGTACACATACGCCCAAGAGCAGCCACTTTTAAGCACTTGCATCGGTACAGAGAATC
ACCATGCTTCAATAGCTTCACATAATCCATGGCGATTTTGCTGATCTTGTTCTTCGCAGT
ATTGACTTGACCAAGAGCGAGCTTGTAGTGATCTTTATTGTAAAGCACATGAAGAAGATC
TTCATAGAAGGGTTGAATTTCCTTGAGGCGAGGAAACTCGTCGATAATGGCAGAGAGCTT
CTCGTTAAAGTTTGATTCTGTGAACTTCACTTTCCGCATGTAGAAGCTACGGAGTTTGCA
AATCCTGTCACCCTTGTGGACAACAGTTGGTGTCTGCCGCTGAGTGCGGGAAAGAACGAT
ATCAACAAACTGTTTTCCGTTTGGCACAACTGTTATCTTCTTGAAATTATATTTCACCAT
TGTTTTTGCTTCTTTAATCTGCACGAAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 25-K104382-0022-097-A03C-8409
GenBank Accession LN497426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K104382-0022-097-A03C-8409
CCATTTACAATTTCGTGCAGATTAAAGAAGCAAAAACAATGGTGAAATATAATTTCAAGA
AGATAACAGTTGTGCCAAACGGAAAACAGTTTGTTGATATCGTTCTTTCCCGCACTCAGC
GGCAGACACCAACTGTTGTCCACAAGGGTGACAGGATTTGCAAACTCCGTAGCTTCTACA
TGCGGAAAGTGAAGTTCACAGAATCAAACTTTAACGAGAAGCTCTCTGCCATTATCGACG
AGTTTCCTCGCCTCAAGGAAATTCAACCCTTCTATGAAGATCTTCTTCATGTGCTTTACA
ATAAAGATCACTACAAGCTCGCTCTTGGTCAAGTCAATACTGCGAAGAACAAGATCAGCA
AAATCGCCATGGATTATGTGAAGCTATTGAAGCATGGTGATTCTCTGTACCGATGCAAGT
GCTTAAAAGTGGCTGCTCTTGGGCGTATGTGTACTGTTATGAAAGGTATTGGTCCTAGTT
TGGCTTATCTTGAACAGGTTAGGCAACACATTGCAAGGCTTCCTTCGATTGATCCAAACA
CTCGAACTCTCTTGATCTGGGAATGTC

Last Updated on 06.12.2018 16:11