Sequences from confirmation amplicon


Confirmation sequences for line 097G01 and hit At4g30340 are shown.
Line ID 097G01
Locus At4g30340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14840879
Primers used for PCR NR61 - 8474, show primer details
   
Sequence Name 68-K102506-0022-097-G01C-8409
GenBank Accession LN511982 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K102506-0022-097-G01C-8409
GCCATTTACATTGAATTATCCTGCAATTAAGGTAAGTAAGTCGGCGAAGACCGATGATGC
AGTGACGATGAGAGGTTGTGGTTTTGCTAATCTTGCCTTGGTTGGTATAGACAAAGAAGA
GCTTCGAGGGAGACTAGCGATGCCTGAGTATCTTCGTATAGCTATGAGAGATTGTATCAA
GAGGAAAGATTCAACAGAGATTTCTGATCATTTGTTACTTCCTGGAGGCGCCGCCGCTGA
TATGGCTCCTCACGCGCCAATGGTTGTGTTTATTAACCCGAAAAGTGGTGGTCGTCATGG
TCCTGTTCTTAAAGAGAGGCTTCAACAATTGATGACTGAAGAACAGGTCTGTTTTTTAAA
AAACAAAAACTTTTTTTTTGTTTGCAACTTAGNAAATGAG
   
Sequence Name 68-K102507-0022-097-G01C-NR61
GenBank Accession LN511983 [GenBank]
Sequencing primer gene specific primer, NR61: 5'-GCTCATTTCATAAGTTGCAAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K102507-0022-097-G01C-NR61
GACCTGTTCTTCGTCATCAATTGTTGAAGCCTCTCTTTAAGAACAGGACCATGACGACCA
CCACTTTTCGGGTTAATAAACACAACCATTGGCGCGTGAGGAGCCATATCAGCGGCGGCG
CCTCCAGGAAGTAACAAATGATCAGAAATCTCTGTTGAATCTTTCCTCTTGATACAATCT
CTCATAGCTATACGAAGATACTCAGGCATCGCTAGTCTCCCTCGAAGCTCTTCTTTGTCT
ATACCAACCAAGGCAAGATTAGCAAAACCACAACCTCTCATCGTCACTGCATCATCGGTC
TTCGCCGACTTACTTACCTTAATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11