Sequences from confirmation amplicon


Confirmation sequences for line 098F01 and hit At5g38410 are shown.
Line ID 098F01
Locus At5g38410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15377639
Primers used for PCR 08ma - 8474, show primer details
   
Sequence Name 56-K114129-0022-098-F01C-08ma
GenBank Accession LN509174 [GenBank]
Sequencing primer gene specific primer, 08ma: 5'-CTATCATCCTTTCAAGTGGACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K114129-0022-098-F01C-08ma
TCTACAACACATGTTACACACTTCACAGTCCTTATAAAGTAAATTTACTTATTATTAAGA
AAATAAGATTTTGTAACTTTTTTCCTCTTAAAACAATGGACAAATATGAATACAAATCCA
TATGTCACTATTATCTATATTTACACAATTTATTCGATTTTTTGTATGTTGTCTTATCTC
ATCGGAAATTTTCAAACTAGTTAACCATCGGAAAAAATCAAGACGCACGGATATATAAAT
TACATATTCATAAATCAGACATTTGACAATCCGATAGAAATTGTCTCATAAAGAGAAAAA
GAACACAGACAATAGGAAATGAAATGAGCAGAGATAATTCATAAGAATGTTTTAGAAAAG
AAATTAAGCTTCGGTGAAGCTTGGGGGCTTGTAGGCAATGAAACTGATGCATTGGACTTG
ACGGGTGTTGTCGAATCCGATGATCCTAATGAAGGCGCCCGGGTACTCCTTCTTGCATTC
TTCAACTTCCTTCAACACTTGATCAATGAAATCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 14-K114128-0022-098-F01C-8409
GenBank Accession LN492595 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114128-0022-098-F01C-8409
GCCATTTACAATTGATTTCATTGATCAAGTGTTGAAGGAAGTTGAAGAATGCACGAAGGA
GTACCCGGGCGCCTTCATTAGGATCATCGGATTCGACAACACCCGTCAAGTCCAATGCAT
CAGTTTCATTGCCTACAAGCCCCCAAGCTTCACCGAAGCTTAATTTCTTTTCTAAAACAT
TCTTATGAATTATCTCTGCTCATTTCATTTCCTATTGTCTGTGTTCTTTTTCTCTTTATG
AGACAATTTCTATCGGATTGTCAAATGTCTGATTTATGAATATGTAATTTATATATCCGT
GCGTCTTGATTTTTTCCGATGGTTAACTAGTTTGAAAATTTCCGATGAGATAAGACAACA
TACAAAAAATCGAATAAATTGTGTAAATATAGATAATAGTGACATATGGATTTGTATTCA
TATTTGTCCATTGTTTTAAGAGGAAAAAAGTTACAAAATCTTATTTTCTTAATAATAAGT
AAATTTACTTTATAAGGACTGTGAAGTGTGTAACATGTGTTTGTAGATCTCAGTCACACA
GAGTTATTACCTTAAAGGAAAAGGATGGTCCACTTGAAAGGATGATA

Last Updated on 06.12.2018 16:11