Sequences from confirmation amplicon


Confirmation sequences for line 098H02 and hit At2g40280 are shown.
Line ID 098H02
Locus At2g40280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16827586
Primers used for PCR BG01 - 8474, show primer details
   
Sequence Name 37-K020131-022-098-H02C-BG01
GenBank Accession LN502452 [GenBank]
Sequencing primer gene specific primer, BG01: 5'-TTCCAATAACAGAAAGTGTTGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K020131-022-098-H02C-BG01
ATTCCTCGTTCTAACGCAAATTGAATCTGAGCTTCATGTTCATCTTTAGGAGCAAACGAC
ATTGTAATGACATCTTTATCTAATAAAGATCCGCCAAAGCTAGCAACACCACAACCAACA
TCTAATACAACCCTTATGTTTTTCCCCCATTTGATACTCGGTAACGCCTGTGAATATGAA
ATGATTTCAACACAAAGATAGATGCTTTCTATTCAAGAAAAACAGGGGAAACAGAGCTAA
GAAAGTGCTGACCTTTTCGATGAACTCAACGTAATGAGTAACCCCGAATTTGAATTGAGT
ACCTCCTCCAGGAAAAACTCAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 38-K020131-022-098-H02C-8409
GenBank Accession LN502830 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K020131-022-098-H02C-8409
TAGATTTCCCGGACATGAAGCCATTTACATTGAGTTTTTCCTGGAGGAGGTACTCAATTC
AAATTCGGGGTTACTCATTACGTTGAGTTCATCGAAAAGGTCAGCACTTTCTTAGCTCTG
TTTCCCCTGTTTTTCTTGAATAGAAAGCATCTATCTTTGTGTTGAAATCATTTCATATTC
ACAGGCGTTACCGAGTATCAAATGGGGGAAAAACATAAGGGTTGTATTAGATGTTGGTTG
TGGTGTTGCTAGCTTTGGCGGATCTTTATTAGATAAAGATGTCATTACAATGTCGTTTGC
TCCTAAAGATGAACATGAAGCTCAGATTCAATTTGCGTTAGAACGAGGAATACCTGCAAC
ACTTTCTGTTATTGGA

Last Updated on 06.12.2018 16:11