Sequences from confirmation amplicon


Confirmation sequences for line 099B04 and hit At1g72650 are shown.
Line ID 099B04
Locus At1g72650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27350035
Primers used for PCR KF05 - 3144, show primer details
   
Sequence Name 01-K103100-0022-099-B04C-KF05
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, KF05: 5'-AGAAGTTCACAGAAGTCAGAAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K103100-0022-099-B04C-KF05
TTTCCCGCGCACTTATTCTCCCAACCAAAATTCTCCTTTACATAACTTGACGATACGAAC
TTTACATACTTTCATACACCCTTTTCGTAACGACTCTCCTTTATAAACTTGATTTTTTCA
CTATTTCATCTACATGCTAACTCACAATTTCGTATTT
   
Sequence Name 01-K103101-0022-099-B04C-3144
GenBank Accession LN486531 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K103101-0022-099-B04C-3144
ATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGCTTTGTGTTCATGTTTTTGG
TGTGAAATTGCACACAGATGCTGTCCAATACTAAAAGAATTGTTTTTTTCTTTGGTAAAG
TTATATGTAGTACTGAAACATGCTTTGGTAGATTGGTTTTTTTACTACTTGAAGAATATA
TTGAGCATATATCAATTTGTTTGCTTTTTCTCTGTTTGGGATTGCAGATACTGTGTTCCT
AAGTTAGATTTGCGATGGGAACTGTGGTTGGAACCGTTGACGACCGCATAGATATGTTTG
AGGGCAATGCTCACACTAGGATCACACCATTTTCTTCAACAAATCAGATTGGAAATCCTG
TTGCCTACAAGCTTGTCCGGGTATGGCTATAAATGTCTTCTTTTCACTGGCTCTCATATA
GTTAATCCTATTGTTTGATTTCTGCGTGTGGCTTCTGACTTCTGTGAACTTC

Last Updated on 06.12.2018 16:11