Sequences from confirmation amplicon


Confirmation sequences for line 099F02 and hit At5g59810 are shown.
Line ID 099F02
Locus At5g59810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24099885
Primers used for PCR ta02 - 8474, show primer details
   
Sequence Name 09-K016200-022-099-F02C-8409
GenBank Accession LN489959 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K016200-022-099-F02C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCGGGAAGTATTTACATTG
AATTTTTTGCGGAAATATTTCAAATCGCTGGATCTCTAATATGACATTGATGTTTTTTGT
AAGAAAATCTTTGTTAAATGTATTTGAACAAAAAAGTAATCTGTGTTTAGTAAAACACAA
CTTGAACCAAACTCAATTAGAGGTAAGTAAAAAGAAAAATTAGAATAATCATTTTTAATG
TTGAATTTCAAACCGAAACAAAAAAATTAGGATTTCATTTGGTACGTACGAGGCTGTTCG
TGGAAATTTTGCGGGACATTGTTTTTTTCCCGGGTTTAAGCGAGGGACTAAAATCTAGGT
TTAAAAAGTATTATTAGTTTCACCCAAATTGAGTTTAGTGCAATGCCCCGGTTACTTACT
ATTGGACTGCCGGTTTACTAGTCCGACCGGCCGGTTTAGTCCGAGTTTTCTTATACACAC
ACCAAGAAACTAACTGCCTTCTCTATTGCAGCCATGAGAATGCAAAAGAAGCCATCTTTT
ACTCATACAAGAGACATATCAATGGCTTCGCCGCAATTCTTGACGAAAATGAAGCTG
   
Sequence Name 10-K016200-022-099-F02C-ta02
GenBank Accession LN490424 [GenBank]
Sequencing primer gene specific primer, ta02: 5'-AGCATCAAAAGCAGAATATGAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K016200-022-099-F02C-ta02
ATGATCTCCTGGCGATTTCTGCAGCTTCATTTTCGTCAAGAATTGCGGCGAAGCCATTGA
TATGTCTCTTGTATGAGTAAAAGATGGCTTCTTTTGCATTCTCATGGCTGCAATAGAGAA
GGCAGTTAGTTTCTTGGTGTGTGTATAAGAAAACTCGGACTAAACCGGCCGGTCGGACTA
GTAAACCGGCAGTCCAATAGTAAGTAACCGGGGCATTGCACTAAACTCAATTTGGGTGAA
ACTAATAATACTTTTTAAACCTAGATTTTAGTCCCTCGCTTAAACCCGGGAAAAAAACAA
TGTCCCGCAAAATTTCCACGAACAGCCTCGTACGTACCAAATGAAATCCTAATTTTTTTG
TTTCGGTTTGAAATTCAACATTAAAAATGATTATTCTAATTTTTCTTTTTACTTACCTCT
AATTGAGTTTGGTTCAAGTTGTGTTTTACTAAACACAGATTACTTTTTTGTTCAAATACA
TTTAACAAAGATTTTCTTACAAAAAACATCAATGTCATATTAGAGATCCAGCGATTTGAA
ATATTTCCGCAAAAAATTCAATGTAAATACTTCCCGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
Primers used for PCR IW29 - 3144, show primer details
   
Sequence Name 02-K033211w-022-099-F02C-IW29
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, IW29: 5'-CTCTCCTGCTTTTGCTCTTAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K033211w-022-099-F02C-IW29
TACTCACTTTCTATATACATTGCTCGTAAGATCGATATGCATTAATTTGTTTCCTACTGT
GTTACAGTCCTACATAGTGTACTTGGGATCTCACGCCCATCTTCCTCAAATCTCTTCTGC
CCATCTCGATGGTGTTGCTCACTCACATCGCACTTTTCTCGCCTCTTTCGTTGGAAGGTA
CCATTACTCCTATTTATGCCAAACCAATGTTTTAAAGATTTGATCAAATAGCAAACCGGG
AAGTATTTCTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGG
GTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGG
ATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTT
TGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCAAT
TCTTTG

Last Updated on 06.12.2018 16:11