Sequences from confirmation amplicon


Confirmation sequences for line 100A02 and hit At5g13150 are shown.
Line ID 100A02
Locus At5g13150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4173947
Primers used for PCR DN37 - 8474, show primer details
   
Sequence Name 10-K101828-0022-100-A02C-8409
GenBank Accession LN490594 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101828-0022-100-A02C-8409
GCCTTTCATTGAATATACGCACCGGAGAAATCAAGAAACCGGATTGTAACAGCGGAGACC
AAACCGGTGAAAAGACGTTTACGGACAGAGGAATGATCTTGATCCGGAAAGACGGCGTTA
CAGAGAGAAAGCTCGCCGGGGAAAAGCACGGTGGAGCAACGGCGAACGATTGAGATCCAC
GAAGCGATTTCACCTTCGAGAGATTCCCAACCTATTCTCTGCACGTCTTCAACGTTGATC
CCTTCGAATCCAACCTCGGTTAGCTCTTCCTTGAACGCGTGCCGTCTCGACATTTCGTAT
GACATACAACACTCTGCTTCGTAACCGGCGGAGATCATCGCTCCGGCGATCTTTTTCAAC
GTCGAGATCGATTCCGGTGAAAAATCATGGAAACTTTCTTCCTCTGCCTCCTCATGGTCT
TGTAAAACGCATCGATCTGAATCATTTGTTGAAGAATTGTTGTGATCGGAATTGCTCCCG
TCGTGGTGGTTGTTGTTGTTGTTGTTCTTCTTCTCCTCCTCTCTG
   
Sequence Name 54-K101831-0022-100-A02C-DN37
GenBank Accession LN508428 [GenBank]
Sequencing primer gene specific primer, DN37: 5'-CAGAGAGGAGGAGAAGAAGAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K101831-0022-100-A02C-DN37
ATTCCGATCCACAATTCTTCAACAAATGATTCAGATCGATGCGTTTTACAAGACCATGAG
GAGGCAGAGGAAGAAAGTTTCCATGATTTTTCACCGGAATCGATCTCGACGTTGAAAAAG
ATCGCCGGAGCGATGATCTCCGCCGGTTACGAAGCAGAGTGTTGTATGTCATACGAAATG
TCGAGACGGCACGCGTTCAAGGAAGAGCTAACCGAGGTTGGATTCGAAGGGATCAACGTT
GAAGACGTGCAGAGAATAGGTTGGGAATCTCTCGAAGGTGAAATCGCTTCGTGGATCTCA
ATCGTTCGCCGTTGCTCCACCGTGCTTTTCCCCGGCGAGCTTTCTCTCTGTAACGCCGTC
TTTCCGGATCAAGATCATTCCTCTGTCCGTAAACGTCTTTTCACCGGTTTGGTCTCCGCT
GTTACAATCCGGTTTCTTGATTTCTCCGGTGCGTATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11