Sequences from confirmation amplicon


Confirmation sequences for line 100B04 and hit At1g15180 are shown.
Line ID 100B04
Locus At1g15180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5224451
Primers used for PCR IW13 - 8474, show primer details
   
Sequence Name 41-K015845-022-100-B04C-8409
GenBank Accession LN503887 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K015845-022-100-B04C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCCGCCGCTGCCGCTTTGC
ACCCGGTGGAGCTTGCATGTTGGTTTCTACGCAGAACTGAGCCGGTTAGGCAGATAATTT
CCATTGAGAACTGAGCCATGTGCACCTTCCCCCCAACACGGTGAGCGACGGGGCAACGGA
GTGATCCACATGGGACTTTTAAACATCATCCGTCGGATGGCGTTGCGAGAGAAGCAGTCG
ATCCGTGAGATCAGCCGACGCACCGGGCAGGCGCGCAACACGATCGCAAAGTATTTGAAC
GCAGGTACATGGCGAACATGGGAGACGCAGAGAGCACCTCCAAAACTAGCTTGCTGCTCC
CGGTGGAAAGAGTTGAAAACGTGACATGGAGAGATCTGCGAGATGGATTATTCACCGCAG
AACTCAAGAGGCTTATCTGCTTTGCCGCTCCTATGGCGGCTGTTGTTATCGCTCAGTTCA
TGTTGCAGATCATCTCAATGGTGATGGTTGGTCACCTCGGAAATCTCTCCCTCGCTAGTG
CCTCTCTCGCCTCCTCCTTCTGCAACGTCACCGGCTTCAGCTTCATCGNCTGTTTTCTCT
TCCTTCTCTCCTCTTTATCTCTATCTATTACTAGTTATCAAATTGCATCCCCTCATTACT
TGATTAGTTGATTGTTACTTTTTGATGCT
   
Sequence Name 42-K015845-022-100-B04C-IW13
GenBank Accession LN504277 [GenBank]
Sequencing primer gene specific primer, IW13: 5'-AACCATATGAGAGTGAGAGGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K015845-022-100-B04C-iw13
CTAATGCAAGACAGAACATTGCTGTGTAAGTCTGAACTCCTACTTTCCGATATAGCTTAG
CTCCATAAGCTTGACCACTCAAAGTATCTAAGGCACATGACAATCCTACCTAGCATCAAA
AAGTAACAATCAACTAATCAAGTAATGAGGGGATGCAATTTGATAACTAGTAATAGATAG
AGATAAAGAGGAGAGAAGGAAGAGAAAACAGACGATGAAGCTGAAGCCGGTGACGTTGCA
GAAGGAGGAGGCGAGAGAGGCACTAGCGAGGGAGAGATTTCCGAGGTGACCAACCATCAC
CATTGAGATGATCTGCAACATGAACTGAGCGATAACAACAGCCGCCATAGGAGCGGCAAA
GCAGATAAGCCTCTTGAGTTCTGCGGTGAATAATCCATCTCGCAGATCTCTCCATGTCAC
GTTTTCAACTCTTTCCACCGGGAGCAGCAAGCTAGTTTTGGAGGTGCTCTCTGCGTCTCC
CATGTTCGCCATGTACCTGCGTTCCAAATACTTTGCGATCGTGTTGCGCGCCTGCCCGGT
GCGTCGGCTGATCTCACGGATCGACTGCTTCTCTCGCAACGCCATCCGACGGATGATGTT
TAAAAGTCCCATGTGGATCACTCCGTTGCCCCGTCGCTCACCGTGTTGGGGGGAAGGTGC
ACATGGCTCANTTCTCAATGGAAATTATCTGCCTAACCGGCT

Last Updated on 06.12.2018 16:11