Sequences from confirmation amplicon


Confirmation sequences for line 100C11 and hit At2g43360 are shown.
Line ID 100C11
Locus At2g43360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18012017
Primers used for PCR HM01 - 8474, show primer details
   
Sequence Name 37-K023014-022-100-C11C-8409
GenBank Accession LN502465 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K023014-022-100-C11C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACCCGGTGAATCTAAGCT
AACTTAAAAGATAACCCACCGAACCGATCATTAATGGTTCTAGAATGAACATTTTTGCAT
GTCAAACTAAAGTGAAAATTTCAAAGTGTACTTGATGTATCCGTGAGCAGAATGAATATG
ATGTTGATGATGGTGTCTGGCTGAATGTGTTTACTAACTTTCTGGGCTCTCACTTTAAAA
CAGAGGCATGGGGATGGAAGTTTGCTGCACCTTAGGCATGATTGAGAAACAACAAGCACT
AGAGCTAAAGAAGGCTGGCCTCACTGCTTATAACCACAATCTTGATACTTCAAGAGAGTA
CTACCCAAACGTCATCACTACTAGAAGTTATGACGATCGCCTTGAAACTCTTAGCCATGT
TCGTGATGCTGGAATCAACGTTTGTTCAGGTAACTTTGATATGAAAATAGTATTCTTGTA
TGCCTCAATTCTATAGAACTAAAGGATTGAAACCTGTCTTCAGCTTGGTCATTAGACTCA
CAGTTTTGTAATATTCGTTGATTTTGATAAATCCATAGATACGACATGTTCTCAAGTGTT
TTCTTTTACCGATAAACAGAGGAATATAAGGCTTGGTGAGG
   
Sequence Name 38-K023014-022-100-C11C-HM01
GenBank Accession LN502841 [GenBank]
Sequencing primer gene specific primer, HM01: 5'-CCTTTCACTGCAAGTAGAGCATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K023014-022-100-C11C-HM01
CTCTCAGGTGAGAAGGAAGTGTTGCCAGCGTGTGTAATAAACCTATTCTGTCTTCCTCTG
CCTCACCAAGCCCTATGATTCCTCCTGTTTATCGGTAAAAGAAAACACTTGAGAACATGT
CGTATCTATGGATTTATCAAAATCAACGAATATTACAAAACTGTGAGTCTAATGACCAAG
CTGAAGACAGGTTTCAATCCTTTAGTTCTATAGAATTGAGGCATACAAGAATACTATTTT
CATATCAAAGTTACCTGAACAAACGTTGATTCCAGCATCACGAACATGGCTAAGAGTTTC
AAGGCGATCGTCATAACTTCTAGTAGTGATGACGTTTGGGTAGTACTCTCTTGAAGTATC
AAGATTGTGGTTATAAGCAGTGAGGCCAGCCTTCTTTAGCTCTAGTGCTTGTTGTTTCTC
AATCATGCCTAAGGTGCAGCAAACTTCCATCCCCATGCCTCTGTTTTAAAGTGAGAGCCC
AGAAAGTTAGTAAACACATTCAGCCAGACACCATCATCAACATCATATTCATTCTGCTCA
CGGATACATCAAGTACACTTTGAAATTTTCACTTTAGTTTGACATGCAAAAATGTTCATT
CTAGAACCATTAATGATCGGTTCGGTGGGTTATCTTTTAAGTTAGCTTAGATTCACCGGG
TTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGA

Last Updated on 06.12.2018 16:11