Sequences from confirmation amplicon


Confirmation sequences for line 101E06 and hit At2g41680 are shown.
Line ID 101E06
Locus At2g41680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17379082
Primers used for PCR GG25 - 8474, show primer details
   
Sequence Name 82-K100901-0022-101-E06C-8409
GenBank Accession LN514156 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K100901-0022-101-E06C-8409
GCCATTTACAATTGAATATATCCTGATTTGCAGTTAAAAAAAAACATCCCGATTTTCTGT
TGGCGACTGTGCCTCACGCGCCTCAGCATTATCTCTTTGCTTCCAAGTCCACAGGCTTAG
ATGATTTTCAGCCGTTGGATATATGTGATGAAGCGACTGAACGGTGGAGATGTGTTTTGA
CTGATTTTACTTCGTCTTTTCAACGGAGGGATAAGCCAATCGCTTTTTTTTTAAGTGGGA
AAGATCGCCAAGAGTTTAAGTGGACGGACGACGCTTTTTATATTGGGCCATAAATGGGCC
TGTTTTGGTACTGAATATTTAGTCACGACGAAGATTCTTAAACCCACCGGAAAATGGGCT
AATCTGTGTGCGGATTGCTTCGCCAAAAAGTTAGTACTAATAATTTATAAAGCATAACTA
GCTAAATGCGAAGACTATATAGTTTACTTAGTTCTGAAGAGGTTGAAGTGGAAATGAAAT
GAACTAGGAGTTGGTGAGGGAAGGTGACAGACTATTTTTTCTTAAGTCAAAAAGTTAAGC
TGACCAAAAAAGAGCAGCCCAATGAAAGCAAGGATCTTTATCTTTAGTCTAGTGTCTGAA
TTCCATGCACTTCCAAT
   
Sequence Name 82-K100907-0022-101-E06C-GG25
GenBank Accession LN514157 [GenBank]
Sequencing primer gene specific primer, GG25: 5'-TGTTGACTAAAACATTGGAAGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K100907-0022-101-E06C-GG25
GATAAGATCTTGCTTTCATTGGGCTGCTCTTTTTTGGTCAGCTTAACTTTTTGACTTAAG
AAAAAATAGTCTGTCACCTTCCCTCACCAACTCCTAGTTCATTTCATTTCCACTTCAACC
TCTTCAGAACTAAGTAAACTATATAGTCTTCGCATTTAGCTAGTTATGCTTTATAAATTA
TTAGTACTAACTTTTTGGCGAAGCAATCCGCACACAGATTAGCCCATTTTCCGGTGGGTT
TAAGAATCTTCGTCGTGACTAAATATTCAGTACCAAAACAGGCCCATTTATGGCCCAATA
TAAAAAGCGTCGTCCGTCCACTTAAACTCTTGGCGATCTTTCCCACTTAAAAAAAAAGCG
ATTGGCTTATCCCTCCGTTGAAAAGACGAAGTAAAATCAGTCAAAACACATCTCCACCGT
TCAGTCGCTTCATCACATATATCCAACGGCTGAAAATCATCTAAGCCTGTGGACTTGGAA
GCAAAGAGATAATGCTGAGGCGCGTGAGGCACAGTCGCCAACAGAAAATCGGGATGTTTT
TTTTTAACTGCAAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATTC

Last Updated on 06.12.2018 16:11