Sequences from confirmation amplicon


Confirmation sequences for line 101G11 and hit At1g58050 are shown.
Line ID 101G11
Locus At1g58050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21480843
Primers used for PCR KS08 - 8474, show primer details
   
Sequence Name 03-K023372-022-101-G11C-8409
GenBank Accession LN487308 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K023372-022-101-G11C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGACATGTAGATTTTTGCCA
TATTGTTAATTGGAAAGGTTTGCTTCAGTAACATCATTGTTGTACTAATAAAGGCCGCTC
AAACATATGTTTTGTTATCATCACTACACCTTTTAAATAATGCAGATGCTGTTATATGGT
GGCATTTTTGGTTGCTTATCACCTATTCTCTCGATTGCTGCTTTCTTGAGCTGCTGCAAA
TCGCCGTTTGTATATGCCAAAGATGAGGTATGTTTATACTTTCTCGGAAACTTGGCTGTT
TTGTGCAAGGAGGTTTTGAACTGACTCTATGTTTGCTCTTCTTTTCTTTCAAGCAAAATG
TGGACCGGGTGAAACTTGCTCTTTTGTCTGATAAGCTGGAGAGTTCGAGTAATTTAAACA
ACAATGATAGACAATCTGATCACCTCCTTATGGTGGTTGCATATGAAAAATGGGTGAGGA
TACTGCATGAGGTATGTGATCAAAGGGTATATCTAGCTGCCACTTACGNNGAAACAGAAT
TNGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGT
   
Sequence Name 04-K023372-022-101-G11C-KS08
GenBank Accession LN487749 [GenBank]
Sequencing primer gene specific primer, KS08: 5'-CTGGTAGACAGTAAGTGGCAGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K023372-022-101-G11C-KS08
CTTTGATCACATACCTCATGCAGTATCCTCACCCATTTTTCATATGCAACCACCATAAGG
AGGTGATCAGATTGTCTATCATTGTTGTTTAAATTACTCGAACTCTCCAGCTTATCAGAC
AAAAGAGCAAGTTTCACCCGGTCCACATTTTGCTTGAAAGAAAAGAAGAGCAAACATAGA
GTCAGTTCAAAACCTCCTTGCACAAAACAGCCAAGTTTCCGAGAAAGTATAAACATACCT
CATCTTTGGCATATACAAACGGCGATTTGCAGCAGCTCAAGAAAGCAGCAATCGAGAGAA
TAGGTGATAAGCAACCAAAAATGCCACCATATAACAGCATCTGCATTATTTAAAAGGTGT
AGTGATGATAACAAAACATATGTTTGAGCGGCCTTTATTAGTACAACAATGATGTTACTG
AAGCAAACCTTTCCAATTAACAATATGGCAAAAATCTACATGTCCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCGGTCGTATTTA
TAGGCGAAAGCAATAAACAAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11