Sequences from confirmation amplicon


Confirmation sequences for line 102E02 and hit At3g09220 are shown.
Line ID 102E02
Locus At3g09220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2828077
Primers used for PCR ND11 - 8474, show primer details
   
Sequence Name 01-K032004-022-102-E02C-8409
GenBank Accession LN486457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K032004-022-102-E02C-8409
TTACAATTGAATATATCCTGGAAGCAGTGTTTCACGACGTTAAAGGGATTTTCACCGCGG
ATTTTCCAGACCAGCCTCCGGTTAAGTTTGATTACACGAACCCGAACGTAACCCAAACAA
ACCCGGGACTTTTGTTCACTCAAAAATCAACTAGCGCCAAGATTTTGAAATTCAATACGA
CGGTGGAGGTAGTGTTACAGAACCATGCGCTTATAGCGGCGGAGAGTCATCCCATGCATC
TACATGGATTTAATTTCCATGTATTGGCTCAAGGGTTTGGTAATTATGACCCGAGCCGTG
ACCGGAGCAAGTTAAATCTCGTTGACCCGCAATCCCGTAACACTTTGGCTGTCCCTGTTG
GTGGATGGGCTGTGATCCGTTTCACTGCTAATAATCCAGGTATAAACTCTCTATCCAATT
CCTTAGTTATACTAAAAATGTAATTATATAGTTTAGAGTTTTACAAAGGCTATTATTGTT
GTTTATGCATGAAACCTAAATATTGGCTACATTGTTTCAGGTGCGTGGATTTTTCATTGT
CATATCGATGTTCACTTACCATTCGGACTAGGGATGATA
   
Sequence Name 02-K032004-0022-102-E02C-ND11
GenBank Accession LN486918 [GenBank]
Sequencing primer gene specific primer, ND11: 5'-TATCATCCCTAGTCCGAATGGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K032004-0022-102-E02C-ND11
CCACGCACCTGAACAATGTAGCCAATATTTAGGTTTCATGCATAAACAACAATAATAGCC
TTTGTAAAACTCTAAACTATATAATTACATTTTTAGTATAACTAAGGAATTGGATAGAGA
GTTTATACCTGGATTATTAGCAGTGAAACGGATCACAGCCCATCCACCAACAGGGACAGC
CAAAGTGTTACGGGATTGCGGGTCAACGAGATTTAACTTGCTCCGGTCACGGCTCGGGTC
ATAATTACCAAACCCTTGAGCCAATACATGGAAATTAAATCCATGTAGATGCATGGGATG
ACTCTCCGCCGCTATAAGCGCATGGTTCTGTAACACTACCTCCACCGTCGTATTGAATTT
CAAAATCTTGGCGCTAGTTGATTTTTGAGTGAACAAAAGTCCCGGGTTTGTTTGGGTTAC
GTTCGGGTTCGTGTAATCAAACTTAACCGGAGGCTGGTCTGGAAAATCCGCGGTGAAAAT
CCCTTTAACGTCGTGAAACACTGCTTCCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
Primers used for PCR FP86 - 8474, show primer details
   
Sequence Name 40-K035336-022-102-E02C-8409
GenBank Accession LN503624 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K035336-022-102-E02C-8409
GCCATGTAGCTTCTTGGGTAACACGAAAGAGTGATTGCTCATAGAAGCTGAGAATTTTGG
ACACGTGGTGTTATCTGCGCATGCCTCTAGCCCAAGACCGATGGTTACGAGCATCTCTTC
GTCCACGTAGCGTGGAACTGGAACCCAGTGAGGTCCATTGACTAAGGCAGTGAGGTTGGA
GTAGAATCTGTAAGC
   
Sequence Name 40-K035336-022-102-E02C-FP86
GenBank Accession LN503625 [GenBank]
Sequencing primer gene specific primer, FP86: 5'-AACGGCTTACAGATTCTACTCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K035336-022-102-E02C-FP86
TGGACCTCCTGGGTTCCGTTCCCGCTACGTGGACGAAGAGATGCTCGTAACCATCGGTCT
TGGGCTAGAGGCATGCGCAGATAACACCACGTGTCCAAAATTCTCAGCTTCTATGAGCAA
TCACTCTTTCGTGTTACCCAAGAAGCTACATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
Primers used for PCR 55xh - 8474, show primer details
   
Sequence Name 51-K119070-0022-102-E02C-55xh
GenBank Accession LN507655 [GenBank]
Sequencing primer gene specific primer, 55xh: 5'-AAATTGTTAATCAGACGACACGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K119070-0022-102-E02C-55xh
GGATAGGACGTTGGTCCTTGTCATTTAAATTGAAACTTCTATATACTTCAGTAGTAATAT
TGACCAAATTAAGTCTTCTATGTTTTAAATTATTATATAGTACTGTGTAATAATATATTA
GTAAACAGGTGAATGGTGGAACACCGATGTAGTCGCGTTAGAGGAGGCAGCAATCGCCAC
CGGAGTTCCTCCAAACAATTCCGACGCTTATACGATCAATGGCCGTCCCGGAAACCTATA
TCCATGCTCCAAAGACAGTGAGTCCAAAGAAACCTAATCTCATGATATTTTTTTCTTAAG
TATAACATACATGGCATTTTTTTTTTTTTTTTTTTTCCCCAACCCCCCCGGCGGGAAAAA
ATTTAAAAATTTAATTCCCCTTCCAAACTTTTTTTTTTGGAAATTACCCTTTTTTTTTTT
TAAGAAAAAAAAAGGGAAGTTTTTCCCCAAACGGGGGGAAAAGAAAAAAAATTTTTTTTT
CCCTTAAAAACCGGGCCCAGAAACTCCCAGTGTTTTTTAAAAAGGCCAATCCCAAAATGG
ATTTTTTTCCCCCCAAACCATTTTAACCCCCACTTTTATTTCCCAAAATTTTTTGTTTTC
CCCCGGGGAAAAACATTGGACCCCTTTTTTTTCCCAAATAAGCCCGCAAACCCCCCCAAA
AAGGGGGGG
   
Sequence Name 19-K119069-0022-102-E02C-8409
GenBank Accession LN494983 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119069-0022-102-E02C-8409
CTTCTTGGGTAACCGAAAGAGTGATTGCTCATAGAAGCTGACCATTTTGGACACATGGTG
TTATCTGCGCATGCCTCTAGCCCAAGACCGATGGTTACGAGCATCTCTTCGTCCACGTAG
CGTGGAACTGGAACCCAGTGAGGTCCATTGACTAAGGCAGTGAGGTTGGAGTAGAATCTG
TAAGCCGTTAACGTGTCGAAGAAGGAAGGTAGTTTCGGCATCAACACGGGCTTTGATCGC
CCTGTTTTCGACGCGCCGCCATAGTGAATGACGCCTCTGGTGGTGGTGTTTGGGAAAGGG
ACGGCGGGAGCGCTGGCGTAAGGGTGAGCTGCCATGTAGTAGGAGGTATCGACGGACTGA
TCGGCGAAGAGAAGGGCGTCGATGGTTTGGCCCGGTGCGATGACAATGACGTCGGTGACG
TAAGGTGCGGTGTAGACTGCGTCGGCGGCTACAACTGTCAGTCTGTGATTGGCTATCTTG
AAAAACAGTTGGATGTTCATGGCCGCGTTTATGATGCGAAGTAAGTATCTCTTTCCTTTC
ACCACGTTTAGGCTAAACATTCCTATTTTCATTCATTAAGAAAATAAAGGTTAATTTGCA
AACTAATAAGTTTGGAATGTGAATTTAATTCTTAATATTTATTCATGCAGCGAAGTTAGA
GAAAAAAAAAAAAAA

Last Updated on 06.12.2018 16:11