Sequences from confirmation amplicon


Confirmation sequences for line 102F03 and hit At2g24010 are shown.
Line ID 102F03
Locus At2g24010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10216981
Primers used for PCR FT32 - 8474, show primer details
   
Sequence Name 89-K100901-0022-102-F03C-8409
GenBank Accession LN514926 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K100901-0022-102-F03C-8409
TTTACAATTGAATATATCTCAGCGTAGCTTTCGGTACAAGGGTCGTACTCGTATTCCTCT
ACCAAAAGCCGTCCATGCAGGAACTTGGTCTGGTTGGTTTGATGTACACATGAGGGTGAG
TAGATGCTGTACCCATTGACTTTGCCAAACTCTCTGTAGGCGAAGTAGAGTGCCCAATTG
CACTTGTCCGAGGTTTTATCCGCAGTGAAGCTGCAGTGTTTGAGGATAGACTTGTAAGTT
TTGTCAGAGATCATTGCGTGTGACCACGCATACATGGCGGCTCCTAATCTGTCGTAATGC
TTGTCCATATCTCCATTTCCCACCTGTGCAAAAGAATATATATGCATTAAGGAGGGATTA
AGGTTT
   
Sequence Name 89-K100907-0022-102-F03C-FT32
GenBank Accession LN514927 [GenBank]
Sequencing primer gene specific primer, FT32: 5'-GAAACCTTAATCCCTCCTTAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K100907-0022-102-F03C-FT32
GAATGGAGATATGGACAGCATTACGACAGATTAGGAGCCGCCATGTATGCGTGGTCACAC
GCAATGATCTCTGACAAAACTTACAAGTCTATCCTCAAACACTGCAGCTTCACTGCGGAT
AAAACCTCGGACAAGTGCAATTGGGCACTCTACTTCGCCTACAGAGAGTTTGGCAAAGTC
AATGGGTACAGCATCTACTCACCCTCATGTGTACATCAAACCAACCAGACCAAGTTCCTG
CATGGACGGCTTTTGGTAGAGGAATACGAGTACGACCCTTGTACCGAAAGCTACGCTGAG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCG
Primers used for PCR FT33 - 3144, show primer details
   
Sequence Name 45-K100996-0022-102-F03C-3144
GenBank Accession LN505459 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K100996-0022-102-F03C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGGAAGCTA
TGCACGCTAATCTTACCTCCATTCCTTATAAGTGGACATTGTGCAATATGGTTGTGAATA
ACAACTGGAAAGATTCCGAGTTTTCAATGTTGCCGATATACAAGGAACTCACTGCCGCTG
GTTTGAGGATCTGGGTCTTTAGGTAAACCATGAAACTAGCTGCAAGTAAAGTACCTTTCG
TGTCCTTTAACCACATTTTGTTGATGTAGTGGCGATACAGACGCAGTGGTTCCAGTGACT
GGGACTCGACTTGCCATCATACACCTTCAA
   
Sequence Name 45-K101000-0022-102-F03C-FT33
GenBank Accession LN505460 [GenBank]
Sequencing primer gene specific primer, FT33: 5'-GATTGAGTTTACTGAGGGCAAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K101000-0022-102-F03C-FT33
CGCCACTACATCAACAAAATGTGGTTAAAGGACACGAAAGGTACTTTACTTGCAGCTAGT
TTCATGGTTTACCTAAAGACCCAGATCCTCAAACCAGCGGCAGTGAGTTCCTTGTATATC
GGCAACATTGAAAACTCGGAATCTTTCCAGTTGTTATTCACAACCATATTGCACAATGTC
CACTTATAAGGAATGGAGGTAAGATTAGCGTGCATAGCTTCCACTGATAGTTTAAACCGA
AGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTC
CTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG
TGGAGATAT

Last Updated on 06.12.2018 16:11