Sequences from confirmation amplicon


Confirmation sequences for line 102G06 and hit At5g65480 are shown.
Line ID 102G06
Locus At5g65480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26177411
Primers used for PCR 15eq - 8474, show primer details
   
Sequence Name 18-K110827-0022-102-G06C-15eq
GenBank Accession LN494356 [GenBank]
Sequencing primer gene specific primer, 15eq: 5'-GCTGATTGATAAGAGTTTTCTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K110827-0022-102-G06C-15eq
TTTTTTTGAAGGAACTTAAAAAGATGGCAGTCTCTTTTCCTCGTCTTCCGAGGTGGTCGT
GTGGTGGTAGTAACACTAAAGATAGAGAGAAGAAAGAGTCAATTCCTAAAGGATCTTCTT
CACTTAGTCCTTCACTGAAGAAGACTACTACTAGTAAGACTGGTTCTTCCTCTTCATCAG
TGAAGAAGACCAAGAGAGGATGGATTGGAAGAGGAGGATCAGGATCAGGAGGGGAAGAGG
ATAAGATTGGGAATGTGGTGTTTCCTGAACCTGATGATCCAGAGTGGTCAATTGGTTGGG
TTGAGCCACATGGACCAGGTTTTAAAGCTGAGGATGATGATTGTGGTGGCTTTGTGGTTT
TGGTTCCTCTTTACAAGAAAGTTATGGATGGTTCAGGGAATCAGATTCCGAATGGCTTCT
TCTCTCCTGCTGCTCCTGGTAATGTTTGTTTCCATTATCATCATCATTCATTAATAGCTA
TCAGGTAAATTATCTGCATGTTCTTTTAAGAACTTAAGTAATTGGTTTGAGTCTTTTGTT
TGTAAATTGATTTGTTCTTTGTTCTGATGACATGTAAACTGCAGATGGGAAGAACATGGA
ACAGTGGCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 02-K110826-0022-102-G06C-8409
GenBank Accession LN487087 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K110826-0022-102-G06C-8409
TTACATTGAATATGCCACTGTTCCATGTTCTTCCCATCTGCAGTTTACATGTCATCAGAA
CAAAGAACAAATCAATTTACAAACAAAAGACTCAAACCAATTACTTAAGTTCTTAAAAGA
ACATGCAGATAATTTACATGATAGCTATTAATGAATGATGATGATAATGGAAACAAACAT
TACCAGGAGCAGCAGGAGAGAAGAAGCCATTCGGAATCTGATTCCCTGAACCATCCATAA
CTTTCTTGTAAAGAGGAACCAAAACCACAAAGCCACCACAATCATCATCCTCAGCTTTAA
AACCTGGTCCATGTGGCTCAACCCAACCAATTGACCACTCTGGATCATCAGGTTCAGGAA
ACACCACATTCCCAATCTTATCCTCTTCCCCTCCTGATCCTGATCCTCCTCTTCCAATCC
ATCCTCTCTTGGTCTTCTTCACTGATGAAGAGGAAGAACCAGTCTTACTAGTAGTAGTCT
TCTTCAGTGAAGGACTAAGTGAAGAAGATCCTTTAGGAATTGACTCTTTCTTCTCTCTAT
CTTTAGTGTTACTACCACCACACAACCACCTCGGAAGACGAGGAAAAGAGACTGCCATCT
TTTTTAATGTTTCCTTTCTAAAAAAAAAACTCCGAACCTCTCCGGAGAAAACTCTTACCA
ATCA

Last Updated on 06.12.2018 16:11