Sequences from confirmation amplicon


Confirmation sequences for line 102H11 and hit At3g04710 are shown.
Line ID 102H11
Locus At3g04710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1278026
Primers used for PCR BW01 - 8474, show primer details
   
Sequence Name 11-K019821-022-102-H11C-BW01
GenBank Accession LN490909 [GenBank]
Sequencing primer gene specific primer, BW01: 5'-ATTCAGGATTTGCTGAACTTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K019821-022-102-H11C-BW01
TAAGACTCCAAAGATGAAACGAAAACAAAAATTATAATTAGAGATTCAAACTACCAAGTG
TCAACTTCAATTCTAATCTACTTATACCAAATTAAAGAAAATTTTCAATTAGTATTTGAG
TTTCTACAGTTCTAGTAATGGCCATAGCCGAAACATTATAGCCAACGAAAATTTCACAAA
AGGAAGAAGAAAAGGCAGCAGAGGAAGAAAAAATCGAAACTTCACCATAAGATCAATTCA
GCTAAAGAAAACTAAGAGAGCCCATCAAGGAATCTATAGTTCATACGCATTCAAAAGACA
GAGATCGATACATCATTCTAAAATATAGAAAAGAGCATACCTGCAAGAGCAGTAGAAGCA
TCAGGAGCCATGGCAGATACTAAGCCCTTCGTTCGTGAAACTTGGAGTGGGTTTTGGATA
TCGGAGTTAGTT
   
Sequence Name 12-K019821-022-102-H11C-8409
GenBank Accession LN491369 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K019821-022-102-H11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACTCCAAAAAAAAACCTCTGGAATCTTCTTAAA
ACCTAGAAGCCTCCGACTCCGACCAACTGAAAAATTAAAAGGCAAAAGCTTTTTTTCAGA
TTTCTGATAATCAGACAGACAGACACAAACAAACACCGGTATCGACGAACGGAACTAACT
CCGATATCCAAAACCCACTCCAAGTTTCACGAACGAAGGGCTTAGTATCTGCCATGGCTC
CTGATGCTTCTACTGCTCTTGCAGGTATGCTCTTTTCTATATTTTAGAATGATGTATCGA
TCTCTGTCTTTTGAATGCGTATGAACTATAGATTCCTTGATGGGCTCTCTTAGTTTTCTT
TAGCTGAATTGATCTTATGGTGAAGTTTCGATTTTTTCTTCCTCTGCTGCCTTTTCTTCT
TCCTTTTGTGAAATTTTCGTTGGCTATAATGTTTCGGCTATGGCCATTACTAGAACTGTA
GAAACTCAAATACTAATTGAAAATTTTCTTTAATTTGGTATAAGTAGATTAGAATTGAAG
TTGACACTTGGTAGTTTGAATCTCTAATTATAATTTTTGTTTTCGTTTCATCTTTGGATT
CTTACTCTAGCGAGGGAGAAAGTTC
Primers used for PCR IW30 - 3144, show primer details
   
Sequence Name 04-K033211w-022-102-H11C-IW30
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, IW30: 5'-CTTTTGAGAGATAGGAAGAAGCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K033211w-022-102-H11C-IW30
CAAAAACACTAGAAATTTTGGAGAAAAGAGATGAAAATTGTGGCTCTGTATATAACTCAC
AAGATGAGAGAAAGAAAAAGAAAAACACCTCTTGTTCATAAGCTGTAATGATGTATACAA
AAAAAAAGATTACAATATGATCTTGGATTGGATAATAAAATGAACTTTTTTCTAGATAAT
CGATTCTTAAACTGAAAATAAGCCCATTTTGATCTAACGTTAGAATGAAAATGAAGAGAT
TTGAGAAGTCAGGGTGTATTTTTTACAAAACGTATTATTGGGTGTATTTTGTAATAGTTA
AATATATGAGGGTCTAAGATTCAAATTTATTAAAAGATATGGATCAATTGTATTTTGTCC
GGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTA
TATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGA
GATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGAT
GCTCCTCGTGGGTGG

Last Updated on 06.12.2018 16:11