Sequences from confirmation amplicon


Confirmation sequences for line 103D08 and hit At2g46070 are shown.
Line ID 103D08
Locus At2g46070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18946877
Primers used for PCR BE06 - 8474, show primer details
   
Sequence Name 32-K028349-022-103-D08C-8409
GenBank Accession LN500448 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K028349-022-103-D08C-8409
TACAATTGAATATATCCTGAAAATGTCTATGAGCTAAAGAACAACTTCCGACACAGACTT
CATGACTGAATATGTCGTTACCCGTTGGTACAGGGCTCCTGAGTTGCTTCTTAACTGCTC
AGAGTACACCGCAGCTATTGATATTTGGTCTGTTGGCTGCATACTCGGCGAAATCATGAC
GGGACAACCGTTGTTTCCAGGCAAAGATTATGTTCATCAGCTTAGGCTTATAACAGAGGT
ATACCAGTAAAGTAAAAAAAGCATCAGTTTTACACTTTTCTTAAACTGCAATGCCCAATT
CTGATTTCACTTAATATCCATTTCTTGATATTAGCTTGTAGGCTCTCCAGACAATTCCAG
CCTCGGCTTCCTTCGCAGTGACAACGCAAGAAGATACGTCAGGCAACTTCCGCGATACCC
GAAACAACAGTTTGCTGCTAGATTCCCGAAAATGCCCACTACGGCTATCGATTTGCTTGA
GAGAATGCTCGTCTTTGATCCTAACCGGCGCATCTCAGGTAACAAACAACACAAAGATCC
TTAAAACATTCATGTGGTTTTTTGCAGTTTGCTTTTGGCTTATCAATTAGACCTGCATGT
GTTGT
   
Sequence Name 32-K028349-022-103-D08C-BE06
GenBank Accession LN500449 [GenBank]
Sequencing primer gene specific primer, BE06: 5'-TCATCGACTACACACACAACACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K028349-022-103-D08C-BE06
GCGCCGGTTAGGATCAAAGACGAGCATTCTCTCAAGCAAATCGATAGCCGTAGTGGGCAT
TTTCGGGAATCTAGCAGCAAACTGTTTGTTTCGGGTATCGCGGAAGTTGCCTGACGTATC
TTCTTGCGTTGTCACTGCGAAGGAAGCCGAGGCTGGAATTGTCTGGAGAGCCTACAAGCT
AATATCAAGAAATGGATATTAAGTGAAATCAGAATTGGGCATTGCAGTTTAAGAAAAGTG
TAAAACTGATGCTTTTTTTACTTTACTGGTATACCTCTGTTATAAGCCTAAGCTGATGAA
CATAATCTTTGCCTGGAAACAACGGTTGTCCCGTCATGATTTCGCCGAGTATGCAGCCAA
CAGACCAAATATCAATAGCTGCGGTGTACTCTGAGCAGTTAAGAAGCAACTCAGGAGCCC
TGTACCAACGGGTAACGACATATTCAGTCATGAAGTCTGTGTCGGAAGTTGTTCTTTAGC
TCATAGACATATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
T
Primers used for PCR IW31 - 3144, show primer details
   
Sequence Name 07-K033211w-022-103-D08C-IW31
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, IW31: 5'-AGCCTTAAGCATCATTTCTCACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K033211w-022-103-D08C-IW31
TTTGCTAGATCTATCAAGTGTTCCTGATCTTCGGGTCAAATGCTCTTTATATATAATTTC
GTTTACTCTAATCTTCAAGCCTCGGTTTGATTCTTTAGGTTATAACCATCAAAGATATTG
TAAGACCTCCGCAACGAGATATCTTCAATGATGTCTACATTGTCTATGAGTTAATGGACA
CTGATCTTCAGCGAATCCTCCGTTCTAACCAAACACTGACCAGTGATCAATGCCGTGTAA
GTCTCTGTGTTGTTCTAATCATTTGTAAAGTCATGGAACTCTCTGATTTGATCTTTATTC
TATTTACTTGTCTTCCTGTTACAGTTCCTAGTATACCAGCTCTTAAGAGGGCTCAAATAC
GTGCACTCGGCCAACATATTACATCGTGATCTAAGGCCAAGCAACGTGCTACTTAACTCG
AAAAACGAGCTAAAGATTGGTGATTTTGGGCTTGCAAGAACAACAATCTGATCCCCGGGT
ACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGAT
AGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTG
AAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG

Last Updated on 06.12.2018 16:11